Li Xiwei, Duan Qingxia, Yu Yamin, Wang Kun, Zhu Hanchuang, Zhang Xue, Liu Caiyun, Jia Pan, Li Zilu, Sheng Wenlong, Zhu Baocun
School of Water Conservancy and Environment, University of Jinan, Jinan, China.
Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China.
Luminescence. 2020 Sep;35(6):941-946. doi: 10.1002/bio.3808. Epub 2020 Apr 23.
Mercury (Hg) is a heavy metal with high toxicity and easy migration; it can be enriched through the food chain, and cause serious threats to the natural environment and human health. So, the development of a method that can be used to detect mercury ions (Hg ) in the environment, in cells, and in organisms is very important. Here, a new 7-hydroxycoumarin-derived carbonothioate-based probe (CC-Hg) was designed and synthesized for detection of Hg . After addition of Hg , a large fluorescence enhancement was observed due to the formation of 7-hydroxyl, which reinforced the intramolecular charge transfer process. The CC-Hg probe had good water solubility and selectivity. Moreover, the probe was able to detect Hg quantitatively over the concentration range 0-2 μM and with a detection limit of 7.9 nM. Importantly, we successfully applied the probe to detect Hg in water samples, in living cells, and in zebrafish. The experimental results demonstrated its potential value in practical applications.
汞（Hg）是一种具有高毒性且易于迁移的重金属；它可通过食物链富集，对自然环境和人类健康造成严重威胁。因此，开发一种可用于检测环境、细胞和生物体中汞离子（Hg ）的方法非常重要。在此，设计并合成了一种基于7-羟基香豆素衍生的碳硫酯的新型探针（CC-Hg）用于检测Hg 。加入Hg 后，由于形成了7-羟基，观察到荧光大幅增强，这增强了分子内电荷转移过程。CC-Hg探针具有良好的水溶性和选择性。此外，该探针能够在0-2 μM的浓度范围内定量检测Hg ，检测限为7.9 nM。重要的是，我们成功地将该探针应用于检测水样、活细胞和斑马鱼中的Hg 。实验结果证明了其在实际应用中的潜在价值。
