Slettjord Tiril H, Sekkenes Hege J, Chi Heng, Bøgwald Jarl, Swain Trilochan, Dalmo Roy A, Kumari Swain Jaya
Norwegian College of Fishery Science, Faculty of Biosciences, Fisheries and Economics, University of Tromsø - The Artic University of Norway, N-9037 Tromsø, Norway.
Cermaq Ltd., Gjærbakknes, N-8286 Steigen, Norway.
Biology (Basel). 2020 Apr 20;9(4):82. doi: 10.3390/biology9040082.
The overexpression of , and may theoretically induce , and expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged , and were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of , and . In addition, the fish were either experimentally challenged with (VA group) or (PS group). The reporter gene (RFP) inserted upstream of the , and genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of and -encoding plasmids, the expression of and was high at the injection site. The spleen expression of , following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The and -overexpressing fish expressed high and mRNA levels in the muscles and on day 4 post-challenge. The expression of in the muscles of fish injected with -encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of , and on both bacterial infections were negligible.
理论上，[具体基因1]、[具体基因2]和[具体基因3]的过表达可能分别诱导[相关基因1]、[相关基因2]和[相关基因3]的表达。这是否也适用于鱼类尚不清楚。编码带有报告基因（RFP）的[具体基因1]、[具体基因2]和[具体基因3]的质粒载体被用作过表达工具，转染到细胞中或进行肌肉注射以监测[相关基因1]、[相关基因2]和[相关基因3]的表达。此外，对鱼类进行实验性攻毒，一组用[病原体1]（VA组），另一组用[病原体2]（PS组）。在[具体基因1]、[具体基因2]和[具体基因3]基因上游插入的报告基因（RFP），在肌肉细胞核和肌肉注射（i.m.）后的炎性细胞中被观察到。PS组：注射编码[具体基因2]和[具体基因3]的质粒后，注射部位[相关基因2]和[相关基因3]的表达较高。注射编码T-bet的质粒后，第2天脾脏中[相关基因1]的表达显著更高。VA组：过表达[具体基因1]和[具体基因2]的鱼在攻毒后第4天肌肉中[相关基因1]和[相关基因2]的mRNA水平较高。注射编码[具体基因3]的质粒的鱼在第7天（攻毒前8天）和第19天（攻毒后4天）肌肉中[相关基因3]的表达显著更高。[具体基因1]、[具体基因2]和[具体基因3]过表达对两种细菌感染的保护作用可忽略不计。
