A continuous-flow enzyme detector for liquid chromatography.

A detection system has been developed for the selective and sensitive detection of enzymes eluting from a liquid chromatographic column. This system monitors a reaction that the enzyme catalyzes and provides a chemical amplification ranging from 10(4) to 10(5). The detection system consists of a reagent or substrate pump, a post-column reactor packed with non-porous spherical glass beads, and a photometric detector. A linear and selective response to a series of enzymes of clinical importance is demonstrated.