Kan X Q, Li Y B, He B, Cheng S, Wei Y, Sun J
Department of Obstetrics and Gynecology, Zhangqiu Maternity and Child Care Hospital, Jinan, China.
Department of Blood Transfusion, Yantai Yuhuangding Hospital, Yantai, China.
J Biol Regul Homeost Agents. 2020 Apr 24;34(2). doi: 10.23812/20-10A.
Recently, important regulatory mechanisms of microRNAs (miRNAs) have been widely reported in human cancers including cervical cancer. The purpose of this study is to preliminarily clarify the function of miR-1294 in cervical cancer. The expression of miR-1294 or FLOT1 was detected using RT-qPCR or Western blot analysis. MTT, Transwell and luciferase reporter assays were used to explore the functional mechanism of miR-1294. The results showed that miR-1294 expression was decreased in cervical cancer. Functionally, overexpression of miR-1294 restrained the viability and metastasis of cervical cancer cells. MiR-1294 can also block EMT and suppress β-catenin expression in cervical cancer cells. Additionally, FLOT1 was confirmed to be a direct target of miR-1294. The knockdown of FLOT1 impeded the progression of cervical cancer. More importantly, miR-1294 inhibited the occurrence of cervical cancer by interacting with FLOT1. In conclusion, miR-1294 inhibits cell viability, migration and invasion by suppressing FLOT1 expression.
最近，微小RNA（miRNA）的重要调控机制在包括宫颈癌在内的人类癌症中被广泛报道。本研究的目的是初步阐明miR-1294在宫颈癌中的作用。采用RT-qPCR或蛋白质免疫印迹分析检测miR-1294或FLOT1的表达。通过MTT、Transwell和荧光素酶报告基因检测来探索miR-1294的功能机制。结果显示，宫颈癌中miR-1294表达降低。在功能上，miR-1294的过表达抑制了宫颈癌细胞的活力和转移。miR-1294还可阻断宫颈癌细胞中的上皮-间质转化（EMT）并抑制β-连环蛋白的表达。此外，FLOT1被证实是miR-1294的直接靶点。FLOT1的敲低阻碍了宫颈癌的进展。更重要的是，miR-1294通过与FLOT1相互作用抑制宫颈癌的发生。总之，miR-1294通过抑制FLOT1表达来抑制细胞活力、迁移和侵袭。
