Coburn Phillip S, Miller Frederick C, Enty Morgan A, Land Craig, LaGrow Austin L, Mursalin Md Huzzatul, Callegan Michelle C
Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
Microorganisms. 2020 Apr 22;8(4):607. doi: 10.3390/microorganisms8040607.
produces many factors linked to pathogenesis and is recognized for causing gastrointestinal toxemia and infections. also causes a fulminant and often blinding intraocular infection called endophthalmitis. We reported that the PlcR/PapR system regulates intraocular virulence, but the specific factors that contribute to virulence in the eye remain elusive. Here, we compared gene expression in ex vivo vitreous humor with expression in Luria Bertani (LB) and Brain Heart Infusion (BHI) broth by RNA-Seq. The expression of several cytolytic toxins in vitreous was less than or similar to levels observed in BHI or LB. Regulators of virulence genes, including PlcR/PapR, were expressed in vitreous. PlcR/PapR was expressed at low levels, though we reported that PlcR-deficient was attenuated in the eye. Chemotaxis and motility genes were expressed at similar levels in LB and BHI, but at low to undetectable levels in vitreous, although motility is an important phenotype for in the eye. Superoxide dismutase, a potential inhibitor of neutrophil activity in the eye during infection, was the most highly expressed gene in vitreous. Genes previously reported to be important to intraocular virulence were expressed at low levels in vitreous under these conditions, possibly because in vivo cues are required for higher level expression. Genes expressed in vitreous may contribute to the unique virulence of endophthalmitis, and future analysis of the virulome in the eye will identify those expressed in vivo, which could potentially be targeted to arrest virulence.
它会产生许多与发病机制相关的因子，并因导致胃肠道毒血症和感染而为人所知。它还会引发一种暴发性且常导致失明的眼内感染，称为眼内炎。我们曾报道PlcR/PapR系统调节眼内毒力，但导致眼部毒力的具体因子仍不清楚。在此，我们通过RNA测序比较了离体玻璃体液中的基因表达与在Luria Bertani（LB）肉汤和脑心浸液（BHI）肉汤中的表达。几种细胞溶解毒素在玻璃体液中的表达低于或类似于在BHI或LB中观察到的水平。包括PlcR/PapR在内的毒力基因调节因子在玻璃体液中表达。PlcR/PapR表达水平较低，尽管我们曾报道PlcR缺陷型在眼部毒力减弱。趋化性和运动性基因在LB和BHI中的表达水平相似，但在玻璃体液中表达水平低至无法检测到，尽管运动性是其在眼部的一个重要表型。超氧化物歧化酶是感染期间眼部中性粒细胞活性的潜在抑制剂，它是玻璃体液中表达最高的基因。在这些条件下，先前报道对眼内毒力重要的基因在玻璃体液中表达水平较低，可能是因为高水平表达需要体内信号。在玻璃体液中表达的基因可能有助于眼内炎独特的毒力，未来对眼部毒力组的分析将鉴定出在体内表达的那些基因，这些基因可能成为阻止毒力的潜在靶点。
