School of Life Sciences, Anhui Agricultural University, Hefei, 230036, P.R. China.
Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Sci Rep. 2020 Apr 24;10(1):6940. doi: 10.1038/s41598-020-63813-y.
Fungal bioremediation is a promising approach to remove heavy-metal from contaminated water. Present study examined the ability of an earthworm gut fungus Trichoderma brevicompactum QYCD-6 to tolerate and remove both individual and multi-metals. The minimum inhibitory concentration (MIC) of heavy metals [Cu(II), Cr(VI), Cd(II) and Zn(II)] against the fungus was ranged 150-200 mg L on composite medium, and MIC of Pb(II) was the highest with 1600 mg L on potato dextrose (PD) medium. The Pb(II) presented the highest metal removal rate (97.5%) which mostly dependent on bioaccumulation with 80.0%, and synchronized with max biomass (6.13 g L) in PD medium. However, on the composite medium, the highest removal rate was observed for Cu(II) (64.5%). Cellular changes in fungus were reflected by TEM analysis. FTIR and solid-state NMR analyses indicated the involvement of different functional groups (amino, carbonyl, hydroxyl, et al.) in metallic biosorption. These results established that the earthworm-associated T. brevicompactum QYCD-6 was a promising fungus for the remediation of heavy-metal wastewater.
真菌生物修复是一种从受污染的水中去除重金属的很有前途的方法。本研究考察了蚯蚓肠道真菌密粘褶菌 QYCD-6 耐受和去除单一和多种金属的能力。重金属 [Cu(II)、Cr(VI)、Cd(II) 和 Zn(II)] 对该真菌的最小抑制浓度 (MIC) 在复合培养基中为 150-200mg/L，而在马铃薯葡萄糖 (PD) 培养基中 Pb(II) 的 MIC 最高，为 1600mg/L。Pb(II) 表现出最高的金属去除率（97.5%），主要依赖于生物积累，为 80.0%，与 PD 培养基中的最大生物量（6.13g/L）同步。然而，在复合培养基中，Cu(II)（64.5%）的去除率最高。真菌的细胞变化通过 TEM 分析反映出来。FTIR 和固态 NMR 分析表明，不同的功能基团（氨基、羰基、羟基等）参与了金属的生物吸附。这些结果表明，与蚯蚓相关的密粘褶菌 QYCD-6 是一种很有前途的用于重金属废水修复的真菌。
