蛋白质中225 - 230纳米正圆二色性带的起源。其在构象分析中的应用。
Origin of the positive 225-230 nm circular dichroism band in proteins. Its application to conformational analysis.
作者信息
Hider R C, Kupryszewski G, Rekowski P, Lammek B
机构信息
Department of Chemistry, University of Essex, Colchester, U.K.
出版信息
Biophys Chem. 1988 Aug;31(1-2):45-51. doi: 10.1016/0301-4622(88)80007-3.
The 225-230 nm circular dichroism band found in many disulfide-containing proteins and peptides is sensitive to environmental changes. This band is assigned to the disulfide bond, the conformation of which influences both the intensity and lambda max of the band. This property can be used to monitor subtle conformation changes observed in many polypeptides. Examples using the alpha-neurotoxins of elapid venoms and neurohypophyseal hormones are discussed.
在许多含二硫键的蛋白质和肽中发现的225 - 230纳米圆二色性带对环境变化敏感。该谱带归因于二硫键，其构象会影响谱带的强度和最大波长。这一特性可用于监测许多多肽中观察到的细微构象变化。文中讨论了使用眼镜蛇科毒液的α-神经毒素和神经垂体激素的示例。
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