Departamento de Farmacia, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta, 36050, Guanajuato, Guanajuato, Mexico.
Facultad de Química, Universidad Autónoma de Yucatán, Calle 43 Calle 90 613 x, Inalámbrica, 97069, Mérida, Yucatán, Mexico.
Inflammopharmacology. 2021 Feb;29(1):295-306. doi: 10.1007/s10787-020-00709-3. Epub 2020 Apr 24.
Cuphea aequipetala Cav (Lythraceae) is an herb used in folk treatment for pain and inflammation. The aim of this study was to evaluate the antinociceptive and anti-inflammatory actions of an ethanol extract from the leaves and stem of Cuphea aequipetala (CAE). The antinociceptive actions of CAE (10-200 mg/kg p.o.) were assessed with the acetic acid-induced writhing, hot plate, and formalin tests. The possible mechanism of action of CAE was evaluated using inhibitors. The effects of CAE on motor coordination were assessed by the rotarod test. The in vitro anti-inflammatory actions of CAE were evaluated using LPS-stimulated primary murine macrophages, and the in vivo anti-inflammatory actions were assessed by the TPA-induced ear oedema and the carrageenan-induced paw oedema tests. The production of inflammatory mediators was estimated from both in vitro and in vivo assays. CAE showed antinociception (ED = 90 mg/kg) in the acetic acid test and in the second phase of the formalin test (ED = 158 mg/kg). Pretreatment with glibenclamide or L-NAME partially reversed the antinociception shown by the plant extract. CAE (50-200 mg/kg) did not affect motor coordination in mice. CAE increased the production of IL-10 in LPS-stimulated macrophages (EC = 10 pg/ml) and, in the carrageenan-induced paw oedema test (threefold increase). In conclusion, CAE induced antinociceptive effects without affecting motor coordination, probably due to the involvement of nitric oxide and ATP-sensitive K channels. CAE also exerts in vitro and in vivo anti-inflammatory effects by increasing the release of IL-10.
紫葳科植物 Cuphea aequipetala Cav（柳叶菜科）是一种民间用于治疗疼痛和炎症的草药。本研究旨在评估 Cuphea aequipetala（CAE）叶和茎的乙醇提取物的镇痛和抗炎作用。通过醋酸诱导的扭体、热板和福尔马林试验评估 CAE（10-200mg/kg，po）的镇痛作用。使用抑制剂评估 CAE 的可能作用机制。通过旋转棒试验评估 CAE 对运动协调的影响。通过 LPS 刺激的原代小鼠巨噬细胞评估 CAE 的体外抗炎作用，通过 TPA 诱导的耳肿胀和角叉菜胶诱导的爪肿胀试验评估 CAE 的体内抗炎作用。从体外和体内测定估计炎症介质的产生。CAE 在醋酸试验和福尔马林试验的第二阶段显示出镇痛作用（ED=90mg/kg）。用格列本脲或 L-NAME 预处理部分逆转了植物提取物的镇痛作用。CAE（50-200mg/kg）对小鼠的运动协调没有影响。CAE 增加了 LPS 刺激的巨噬细胞中 IL-10 的产生（EC=10pg/ml），并在角叉菜胶诱导的爪肿胀试验中（增加三倍）。总之，CAE 诱导了镇痛作用而不影响运动协调，这可能是由于一氧化氮和 ATP 敏感性 K 通道的参与。CAE 还通过增加 IL-10 的释放发挥体外和体内抗炎作用。
