Zelikman I, Hjertén S
Institute of Biochemistry, University of Uppsala, Swden.
Biomed Chromatogr. 1988 Nov;2(6):245-8. doi: 10.1002/bmc.1130020604.
A new cost-effective method for determining the amount of total propranolol (free and protein-bound) in plasma samples and the amount of the drug in tablets and injection solutions has been developed. The method is based on cation-exchange chromatography at pH 8.5 on an inexpensive column of agar, the native sulfate (and carboxylic) groups of which interact with the positively charged propranolol (this interaction is reinforced by the 'aromatic adsorption' characteristic of the agar matrix). The method can be used for quantitative analyses at both low (1 x 10(-2) mg/L) and high (5 x 10(3) mg/L) concentrations of the drug. For analyses of plasma and tablets--but not for injection solutions--extraction of the propranolol is required. The extraction time was 6 min. On a 6(ID) mm x 9 mm agar column the run time was less than 10 min.
已开发出一种经济高效的新方法，用于测定血浆样品中总普萘洛尔（游离和与蛋白质结合的）含量以及片剂和注射溶液中的药物含量。该方法基于在pH 8.5条件下，在廉价的琼脂柱上进行阳离子交换色谱法，琼脂柱的天然硫酸根（和羧基）基团与带正电荷的普萘洛尔相互作用（琼脂基质的“芳香吸附”特性增强了这种相互作用）。该方法可用于低浓度（1×10⁻²mg/L）和高浓度（5×10³mg/L）药物的定量分析。对于血浆和片剂的分析——但不适用于注射溶液——需要提取普萘洛尔。提取时间为6分钟。在6（内径）mm×9mm的琼脂柱上，运行时间不到10分钟。
