State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 30045, China; Tianjin Institute of Environmental and Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin 300050, China.
Tianjin Institute of Environmental and Operational Medicine, Key Laboratory of Risk Assessment and Control for Environment & Food Safety, Tianjin 300050, China.
Sci Total Environ. 2020 Jul 15;726:138500. doi: 10.1016/j.scitotenv.2020.138500. Epub 2020 Apr 6.
The health risks of exposure to plasticizers have received widespread attention, however, little is known about the effects of fast food packaging plasticizers on steroid hormone synthesis. In the present study, the types and migration of plasticizers in some commonly used fast-food packaging materials were detected by GC-MS, and the interference effects of these plasticizers and their metabolites on steroid hormone synthesis in the human body were evaluated by the H295R steroidogenesis assay. The GC-MS results showed that the main plasticizer compounds that migrated from fast food packaging into food were di(2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bis(2-ethylhexyl) adipate (DEHA). Exposure to these chemicals (100-1000 μM) can significantly reduce the viability of H295R cells in a dose-response manner, and these plasticizers and their metabolites that migrated into oily foods at high temperatures (0.25-25 μM) could significantly increase the E2 level and reduce the T level in H295R cells. According to the qRT-PCR data, 0.25 to 25 μM mono(2-ethylhexyl) phthalate (MEHP) significantly upregulated the expression levels of 17β-HSD1 and CYP19A1, and downregulated those of CYP17A1, CYP11A1 and StAR. The Western blot results were consistent with those of qRT-PCR. In summary, these results indicated that even exposure to low concentrations (≤1 mg/l or 2.5 μM) of these chemicals and their metabolites can cause significant endocrine-disrupting effects.
接触增塑剂对健康的危害已引起广泛关注，然而，关于快餐包装塑料增塑剂对甾体激素合成的影响知之甚少。本研究采用 GC-MS 检测了一些常用快餐包装材料中增塑剂的类型和迁移情况，并通过 H295R 甾体生成测定法评价了这些增塑剂及其代谢物对人体甾体激素合成的干扰作用。GC-MS 结果表明，从快餐包装迁移到食品中的主要增塑剂化合物为邻苯二甲酸二（2-乙基己基）酯（DEHP）、邻苯二甲酸二丁酯（DBP）和己二酸二（2-乙基己基）酯（DEHA）。这些化学物质（100-1000μM）暴露以剂量反应方式显著降低 H295R 细胞的活力，而这些在高温下（0.25-25μM）迁移到油性食品中的增塑剂及其代谢物可显著增加 H295R 细胞中的 E2 水平并降低 T 水平。根据 qRT-PCR 数据，0.25 至 25μM 单（2-乙基己基）邻苯二甲酸酯（MEHP）显著上调 17β-HSD1 和 CYP19A1 的表达水平，下调 CYP17A1、CYP11A1 和 StAR 的表达水平。Western blot 结果与 qRT-PCR 结果一致。总之，这些结果表明，即使接触这些化学物质及其代谢物的低浓度（≤1mg/l 或 2.5μM）也会导致明显的内分泌干扰作用。
