人柯萨奇-腺病毒受体跨膜和胞质结构域中的天然变异对病毒内化没有影响。

Naturally occurring variants in the transmembrane and cytoplasmic domains of the human Coxsackie- and adenovirus receptor have no impact on virus internalisation.

机构信息

Clinic for General and Interventional Cardiology/Angiology, Herz- und Diabeteszentrum NRW, Ruhr-Universität Bochum, 32545, Bad Oeynhausen, Germany; Proteom- und Metabolomforschung, Fakultät für Biologie & CeBiTec, Universität Bielefeld, 33501, Bielefeld, Germany.

Virologie, Friedrich-Alexander-Universität Erlangen-Nürnberg, Schlossgarten 4, 91054, Erlangen, Germany.

出版信息

Biochem Biophys Res Commun. 2020 Jun 25;527(2):401-405. doi: 10.1016/j.bbrc.2020.03.082. Epub 2020 Apr 23.

DOI:10.1016/j.bbrc.2020.03.082

PMID:32334832

Abstract

The Coxsackie- and adenovirus receptor (CAR) mediates homophilic cell-cell contacts and susceptibility to both human pathogenic viruses through its membrane-distal immunoglobulin domain. In the present study, we screened five missense variants of the human CAR gene for their influence on adenovector or Coxsackievirus entry into Chinese hamster ovary cells. The CAR variants facilitated virus internalisation to a similar extent as wild type CAR. This underlines CAR's presumed invariance and essential physiological role in embryogenesis.

摘要

柯萨奇-腺病毒受体 (CAR) 通过其膜远端免疫球蛋白结构域介导同种细胞间的接触，并通过其膜远端免疫球蛋白结构域易感性导致人类致病病毒。在本研究中，我们筛选了人 CAR 基因的五个错义变体，以研究它们对腺病毒或柯萨奇病毒进入中国仓鼠卵巢细胞的影响。CAR 变体促进病毒内化的程度与野生型 CAR 相同。这强调了 CAR 在胚胎发生中的假定不变性和重要生理作用。