新型甜菜碱-氨基酸基天然深共晶溶剂用于增强玉米芯的酶解。
Novel betaine-amino acid based natural deep eutectic solvents for enhancing the enzymatic hydrolysis of corncob.
机构信息
Key Laboratory of Low Carbon Energy and Chemical Engineering, College of Chemistry and Bioengineering, Shandong University of Science and Technology, Qingdao 277590, China.
State Key Laboratory of Heavy Oil Processing, China University of Petroleum (East China), Qingdao 266580, China.
出版信息
Bioresour Technol. 2020 Aug;310:123389. doi: 10.1016/j.biortech.2020.123389. Epub 2020 Apr 18.
A novel natural deep eutectic solvent (NDES) with water content ranging from 65 to 93 wt%, in which betaine (Bet) acts as the cation and amino acids (AAs) as the anions, was prepared by a simple and green chemical route. [Bet][AA] NDES showed excellent xylan and lignin solubility, however, scare cellulose solubility. A mild and facile pretreatment process with [Bet][AA] NDES was carried out at 60 °C for 5 h. The enzymatic hydrolysis efficiency of cellulose and corncob was significantly improved. Detailed characterization showed that the enhancement of cellulose digestibility derived mainly from xylan and lignin removal. Xylan and lignin removal for [Bet][Lys]-W87 was 47.68 and 49.06%, while it was 42.20% and 57.01% for [Bet][Arg]-W82, respectively. FT-IR, SEM, XRD, and HSQC NMR studies confirmed the effectiveness and mechanism of [Bet][Lys]-W87 and [Bet][Arg]-W82 on biomass pretreatment.
一种新型的天然深共熔溶剂（NDES），含水量在 65 到 93wt%之间，其中甜菜碱（Bet）作为阳离子，氨基酸（AAs）作为阴离子，通过简单而绿色的化学途径制备。[Bet][AA] NDES 表现出优异的木聚糖和木质素溶解能力，但对纤维素的溶解能力较差。采用温和、简便的预处理工艺，在 60°C 下处理 5 小时。[Bet][AA] NDES 显著提高了纤维素和玉米芯的酶解效率。详细的表征表明，纤维素消化率的提高主要源于木聚糖和木质素的去除。[Bet][Lys]-W87 对木聚糖和木质素的去除率分别为 47.68%和 49.06%，而[Bet][Arg]-W82 对木聚糖和木质素的去除率分别为 42.20%和 57.01%。FT-IR、SEM、XRD 和 HSQC NMR 研究证实了[Bet][Lys]-W87 和[Bet][Arg]-W82 对生物质预处理的有效性和作用机制。
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