Khrestchatisky M, Djabali M, Thouveny Y, Fontes M
Laboratoire de Biologie de la Différenciation Cellulaire, C.N.R.S., U.A. 179, Faculté des Sciences de Luminy, Marseille, France.
Cell Differ Dev. 1988 Dec;25(3):203-12. doi: 10.1016/0922-3371(88)90117-7.
Amputation of the tail of the amphibian Pleurodeles waltlii leads to the formation of a blastema composed of undifferentiated cells during the first stages of regeneration. Between the 3rd and 4th week following amputation, the first fully differentiated striated muscle cells appear, and in the 6th week myogenic differentiation extends throughout the regenerate. Myoblasts appear in cell patches where cells fuse as differentiation proceeds. Using a cloned cDNA probe to a striated muscle actin gene specifically expressed in adult skeletal muscle, we show that no corresponding mRNA remains during dedifferentiation. A new actin transcript of the same length as cardiac actin transcript appears in the 4th week after amputation. We conclude that for the myogenic cell line, differentiation in the regenerate is accompanied by an actin isoform transition similar to that seen in embryonic terminal differentiation of higher vertebrates.
切除两栖动物肋突螈的尾巴后，在再生的最初阶段会形成由未分化细胞组成的芽基。截肢后的第3至4周，第一批完全分化的横纹肌细胞出现，到第6周，生肌分化扩展至整个再生组织。随着分化的进行，成肌细胞出现在细胞融合的细胞斑块中。我们使用针对成年骨骼肌中特异性表达的横纹肌肌动蛋白基因的克隆cDNA探针，发现去分化过程中没有相应的mRNA残留。截肢后第4周出现了一种与心肌肌动蛋白转录本长度相同的新肌动蛋白转录本。我们得出结论，对于生肌细胞系，再生组织中的分化伴随着肌动蛋白异构体的转变，这与高等脊椎动物胚胎终末分化中观察到的情况相似。
