Laboratory of Bioinformatics, Institute of Microbiology, Czech Academy of Sciences, v.v.i., Videnska 1083, Prague, 14220, Czech Republic.
Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, v.v.i., Flemingovo n. 2, Prague, 16610, Czech Republic.
Proteomics. 2020 Jul;20(14):e2000032. doi: 10.1002/pmic.202000032. Epub 2020 Jun 29.
In this paper, correlation analysis of protein and mRNA levels in the soil dwelling bacteria Streptomyces coelicolor (S. coelicolor M145) is presented during development of the population as it grew in liquid medium using three biological and two technical replicates, measured during exponential growth, and its entry into the stationary phase. The proteome synthesis time series are compared with the gene expression time series measured previously under identical experimental conditions. Results reveal that about one third of protein/mRNA synthesis profiles are well correlated while another third are correlated negatively. Functional analysis of the highly correlated groups is presented. Based on numerical simulation, the negative correlation between protein and mRNA is shown to be caused by the difference between the rate of translation and protein degradation.
本文介绍了在液体培养基中生长的土壤栖居菌变铅青链霉菌（Streptomyces coelicolor M145）的种群发育过程中，在三个生物重复和两个技术重复的条件下，利用同步生长和进入静止期的方法，对其蛋白和 mRNA 水平进行相关分析。将蛋白质合成时间序列与先前在相同实验条件下测量的基因表达时间序列进行了比较。结果表明，大约三分之一的蛋白/ mRNA 合成谱呈正相关，而另外三分之一呈负相关。对高度相关的群组进行了功能分析。通过数值模拟，显示了蛋白和 mRNA 之间的负相关是由翻译和蛋白降解的速度差异引起的。
