Kiyota Kyohei, Yoshimitsu Masato, Kajimura Keiji, Yamano Tetsuo
Osaka Institute of Public Health.
Shokuhin Eiseigaku Zasshi. 2020;61(1):17-21. doi: 10.3358/shokueishi.61.17.
Oranges are consumed worldwide; however, they contain Cit s 2, a major profilin allergen. We aimed to reduce Cit s 2 levels by preparing mixed orange fresh juice with pineapple, as a convenient method for any kitchen. Cit s 2 levels in orange extracts digested with pineapple extract and its protease bromelain were evaluated with quantitative enzyme-linked immunosorbent assay. Cit s 2 levels decreased according to reaction temperature and time, which was inhibited by iodoacetic acid. Treatment with pineapple extract diluted 40-fold and 0.1 mg/mL of bromelain at 37℃ for 30 min contributed to reducing residual Cit s 2 levels below the cut-off of 15%, respectively. Since this condition can increase the proportion of orange juice and reduce the risk of ingesting the pineapple allergen bromelain, it is considered to be more practical. Broad utilization of proteases in hypoallergenic food products is expected following clinical studies for verification.
橙子在全球范围内都有消费；然而，它们含有主要的肌动蛋白结合蛋白过敏原Cit s 2。我们旨在通过将橙子与菠萝混合制备新鲜果汁，作为一种适合任何厨房的便捷方法来降低Cit s 2的水平。用定量酶联免疫吸附测定法评估了用菠萝提取物及其蛋白酶菠萝蛋白酶消化后的橙子提取物中Cit s 2的水平。Cit s 2水平根据反应温度和时间而降低，这被碘乙酸抑制。在37℃下用40倍稀释的菠萝提取物和0.1 mg/mL的菠萝蛋白酶处理30分钟，分别有助于将残留的Cit s 2水平降低到15%的临界值以下。由于这种条件可以增加橙汁的比例并降低摄入菠萝过敏原菠萝蛋白酶的风险，因此被认为更具实用性。在经过临床研究验证后，有望在低过敏性食品中广泛使用蛋白酶。
