Yamashita Azusa, Shinohara Yuji, Sakai Hiroaki, Miyamoto Yasuhisa, Suzuki Koji, Nagatomi Yasushi
Food Safety Laboratories, Asahi Quality & Innovations, Ltd.
Shokuhin Eiseigaku Zasshi. 2020;61(1):41-46. doi: 10.3358/shokueishi.61.41.
DNA from hair that has undergone retort pouch sterilization shows considerably more fragmentation. Assessing the extent of DNA fragmentation using a PCR assay could therefore be applied to infer the sterilization history of a contaminated food sample. Such assessments could make it possible to determine whether a food sample had been contaminated by hair during the production process. To determine the extent of retort pouch sterilization, primers specific for the detection of human DNA were designed to give an amplification product of approx 500 bp. Hair was subjected to retort sterilization as a model of contamination, and PCR was performed using the extracted DNA as a template and the primer set for determining retort pouch sterilization. The results showed that no DNA amplification occurred in retort pouch samples, whereas amplification was observed in samples that were unheated, heated in hot water, or heated in a microwave oven. The present method was thus able to specifically detect thermal decomposition of DNA from hair that had undergone retort sterilization, and will be useful for determining whether hair discovered in a retort pouch was responsible for contamination.
经过蒸煮袋灭菌处理的毛发中的DNA显示出更多的片段化。因此，使用聚合酶链式反应（PCR）检测法评估DNA片段化程度可用于推断受污染食品样本的灭菌历史。此类评估能够确定食品样本在生产过程中是否被毛发污染。为确定蒸煮袋灭菌程度，设计了用于检测人类DNA的特异性引物，以产生约500碱基对的扩增产物。将毛发作为污染模型进行蒸煮灭菌处理，以提取的DNA为模板，使用用于确定蒸煮袋灭菌的引物对进行PCR。结果表明，蒸煮袋样本中未发生DNA扩增，而在未加热、热水加热或微波炉加热的样本中观察到了扩增。因此，本方法能够特异性检测经过蒸煮灭菌的毛发中DNA的热分解情况，对于确定在蒸煮袋中发现的毛发是否导致污染将很有用处。
