Mardin Artuklu University, Mardin, Turkey.
Sutcu Imam University, Kahramanmaraş, Turkey.
Folia Med (Plovdiv). 2020 Mar 31;62(1):70-75. doi: 10.3897/folmed.62.e47735.
2-AAF and DEN are well-known liver toxicants commonly used to stimulate tumors in laboratory animals.
The aim of this study was to investigate the effect of octreotide on DEN-induced and 2-AAF-supplemented hepatocarcinogenesis in Wistar albino rats.
In this study, 64 Wistar albino rats were divided into 8 groups. DEN (175 mg/kg) initiated and 2-AAF (20 mg/kg) promoted liver carcinogenesis in rats. The tumor growth inhibitor octreotide (300 μg/kg) was used. Rats were sacrificed at the end of experiment and their liver tissues were taken for the study. SOD, GSH-Px, CAT activities, NO and MDA levels were measured spectrophotometrically. Also, Hsp70 and 8-OHdG was measured by the ELISA method.
In group 7, MDA, 8-OHdG, and Hsp70 levels were significantly increased. In addition, SOD, GSH-Px activity was significantly reduced in this group. MDA, 8-OHdG and Hsp70 levels were significantly reduced in Group 8, which received octreotide for treatment.
DEN and 2-AAF cause very serious liver damage. Octreotide protects the liver from carcinogenesis, increases the activity of cellular antioxidant enzymes and helps reduce DNA damage. Therefore, octreotide may be an inhibitor in tumor cells and may reduce oxidative stress.
2-AAF 和 DEN 是众所周知的肝毒性物质，常用于刺激实验动物的肿瘤。
本研究旨在探讨奥曲肽对 DEN 诱导和 2-AAF 补充的 Wistar 白化大鼠肝癌发生的影响。
本研究将 64 只 Wistar 白化大鼠分为 8 组。用 DEN（175mg/kg）启动和 2-AAF（20mg/kg）促进大鼠肝癌发生。使用肿瘤生长抑制剂奥曲肽（300μg/kg）。实验结束时处死大鼠，取其肝组织进行研究。用分光光度法测定 SOD、GSH-Px、CAT 活性、NO 和 MDA 水平。还通过 ELISA 法测定 Hsp70 和 8-OHdG。
在第 7 组中，MDA、8-OHdG 和 Hsp70 水平显著升高。此外，该组 SOD、GSH-Px 活性显著降低。第 8 组接受奥曲肽治疗，MDA、8-OHdG 和 Hsp70 水平显著降低。
DEN 和 2-AAF 可导致严重的肝损伤。奥曲肽可保护肝脏免受致癌作用，增加细胞抗氧化酶的活性，并有助于减少 DNA 损伤。因此，奥曲肽可能是肿瘤细胞的抑制剂，可降低氧化应激。
