Branca M, Garcovich A, Linfante L D, Macrì A, Mantovani A, Olivetti G, Salvatore G
Istituto Superiore di Sanità, Università Cattolica del S. Cuore, Roma, Italy.
Contact Dermatitis. 1988 Nov;19(5):320-34. doi: 10.1111/j.1600-0536.1988.tb02946.x.
This paper explores the irritancy potential of n-alkyl benzoate preservatives (methyl benzoate, MB; ethyl benzoate, EB; n-propyl benzoate, n-PB; n-butyl benzoate, n-BB), allowable in cosmetic formulations. Comparative experiments were carried out involving repeated daily application for all compounds (up to maximum of 6 days) on clipped rabbit skin in 2 distinct regions: the dorsum (0.5 ml per treatment) and the external surface of the outer ear (0.2 ml per treatment). According to the different anatomo-physiological features, benzoates elicited dissimilar reactions in the dorsum and the external ear. In the first mentioned area, there were widespread inflammatory reactions accompanied, in some cases, by necrotic changes and slight epidermal hyperplasia; on the other hand, hyperplasia was the most prominent and earliest alteration in the ear. The irritancy potential was closely related to the duration of treatment as well to the increase in the molecular weight of benzoates.
本文探讨了化妆品配方中允许使用的苯甲酸正烷基酯类防腐剂（苯甲酸甲酯，MB；苯甲酸乙酯，EB；苯甲酸正丙酯，n-PB；苯甲酸正丁酯，n-BB）的潜在刺激性。进行了对比实验，对所有化合物在兔背部皮肤的两个不同区域（背部每次处理0.5毫升，耳外表面每次处理0.2毫升）进行每日重复涂抹（最长6天）。根据不同的解剖生理特征，苯甲酸酯类在背部和耳部引发了不同的反应。在上述第一个区域，出现了广泛的炎症反应，在某些情况下伴有坏死变化和轻微的表皮增生；另一方面，增生是耳部最突出和最早出现的变化。潜在刺激性与处理时间以及苯甲酸酯类分子量的增加密切相关。
