卡罗琳斯卡学院人类胚胎干细胞库

Karolinska Institutet Human Embryonic Stem Cell Bank.

作者信息

Main Heather, Hedenskog Mona, Acharya Ganesh, Hovatta Outi, Lanner Fredrik

机构信息

Department of Clinical Sciences, Intervention and Technology, Karolinska Insitutet, 17177, Stockholm, Sweden; Gynecology and Reproductive Medicine, Karolinska University Hospital, 14186 Stockholm, Sweden; Ming Wai Lau Center for Reparative Medicine, Stockholm node, Karolinska Institutet, 17177, Stockholm, Sweden.

出版信息

Stem Cell Res. 2020 May;45:101810. doi: 10.1016/j.scr.2020.101810. Epub 2020 Apr 21.

DOI:10.1016/j.scr.2020.101810

PMID:32339905

Abstract

The Karolinska Institutet Human Embryonic Stem Cell Bank (KI Stem Cell Bank) was established at KI, Stockholm, Sweden, when the first human embryonic stem cell (hESC) line was derived by Professor Hovatta and colleagues in 2002. Since then, the bank has grown to include 60 hESC lines. From the very beginning the aim of the bank has been derivation of hESC lines suitable for clinical use. Step by step progress has been made towards this goal, including removal of xeno components, establishment of chemically defined conditions and Good Manufacturing Practice (GMP) compliancy. Today our bank includes such clinical grade hESC line, KARO1, derived and banked according to GMP guidelines. Many of the hESC lines in the bank have been distributed to the scientific community and are deposited in the Stockholm Medical Biobank available for research on collaborative basis.

摘要

卡罗林斯卡学院人类胚胎干细胞库（KI干细胞库）于2002年在瑞典斯德哥尔摩的卡罗林斯卡学院成立，当时霍瓦塔教授及其同事首次成功分离出人类胚胎干细胞（hESC）系。从那时起，该库已发展到包含60个hESC系。从一开始，该库的目标就是分离出适合临床应用的hESC系。为实现这一目标已逐步取得进展，包括去除异种成分、建立化学成分明确的条件以及符合药品生产质量管理规范（GMP）。如今，我们的库中包含这样一个临床级hESC系——KARO1，它是按照GMP指南分离并保存的。该库中的许多hESC系已分发给科学界，并存放在斯德哥尔摩医学生物样本库中，可供合作研究使用。