Izui S, Lambert P H, Fournié G J, Türler H, Miescher P A
J Exp Med. 1977 May 1;145(5):1115-30. doi: 10.1084/jem.145.5.1115.
After injection of lipopolysaccharides (LPS) in mice, there is first a release of DNA into plasma and secondly an induction of anti-DNA antibodies. The circulating DNA was purified from plasma and physico-immunochemically characterized. This DNA has a similar density to mammalian cellular DNA,is 4--6S insize, and probably represents a mixture of single-stranded DNA (SSDNA) and double-stranded DNA (DSDNA) or DSDNA with some single-stranded regions. This purified DNA was shown to react with anti-DNA antibodies which appeared as early as 3 days after a single injection of LPS in mice. In serum, DNA-anti-DNA complexes were not detected, although unidentified circulating immune complex-like material was demonstrated 5-8 days after the injection of LPS. In tissues, particularly in renal glomeruli, fine granular immune complex-type immunoglobulin deposits appeared along the glomerular capillary walls and in the mesangium 3 days after the injection of LPS. There is a direct correlation between the level of anti-DNA antibodies and the intensity of glomerular deposits and about 40% of immunoglobulins eluted from kidneys are anti-DNA antibodies, indicating that some of the immune complexes localized in kidneys are DNA-anti-DNA complexes. Based on these observations, the following hypothetical mechanism for the glomerular localization of DNA-anti-DNA complexes after the injection of LPS in mice is proposed. First, DNA, which has been released in circulating blood after injection of LPS, might bind to renal glomeruli, probably on glomerular basement membranes (GBM) through a high affinity of GBM for DNA; secondly, circulating anti-DNA antibodies, which appear later, might react with the glomerular-bound DNA and form immune complexes independently of circulating immune complexes. However, the possibility of direct deposition of immune complexes is not ruled out.
给小鼠注射脂多糖（LPS）后，首先会有DNA释放到血浆中，其次会诱导抗DNA抗体产生。从血浆中纯化出循环DNA，并对其进行物理免疫化学特性分析。这种DNA的密度与哺乳动物细胞DNA相似，大小为4 - 6S，可能代表单链DNA（SSDNA）和双链DNA（DSDNA）的混合物，或者是带有一些单链区域的DSDNA。已证明这种纯化的DNA能与抗DNA抗体反应，这些抗体在给小鼠单次注射LPS后3天就会出现。在血清中，未检测到DNA - 抗DNA复合物，尽管在注射LPS后5 - 8天发现了未明确的循环免疫复合物样物质。在组织中，特别是在肾小球中，注射LPS后3天，沿肾小球毛细血管壁和系膜出现了细颗粒状免疫复合物型免疫球蛋白沉积。抗DNA抗体水平与肾小球沉积物强度之间存在直接相关性，从肾脏洗脱的免疫球蛋白中约40%是抗DNA抗体，这表明定位在肾脏中的一些免疫复合物是DNA - 抗DNA复合物。基于这些观察结果，提出了以下关于给小鼠注射LPS后DNA - 抗DNA复合物在肾小球定位的假说机制。首先，注射LPS后释放到循环血液中的DNA可能通过肾小球基底膜（GBM）对DNA的高亲和力与肾小球结合，可能是在肾小球基底膜上；其次，随后出现的循环抗DNA抗体可能与肾小球结合的DNA反应并形成免疫复合物，而与循环免疫复合物无关。然而，不排除免疫复合物直接沉积的可能性。
