五倍子葡萄糖、鞣花酸和没食子酸与牛血清白蛋白相互作用的研究：光谱分析。
Studies on interactions of pentagalloyl glucose, ellagic acid and gallic acid with bovine serum albumin: A spectroscopic analysis.
机构信息
Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China.
Key Lab. of Biomass Energy and Material, Nanjing, Jiangsu Province 210042, China; Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, China; Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Nanjing Forestry University, Nanjing 210042, China.
出版信息
Food Chem. 2020 Sep 15;324:126872. doi: 10.1016/j.foodchem.2020.126872. Epub 2020 Apr 20.
Fluorescence techniques and circular dichroism (CD) were utilized to investigate the interactions of a typical gallotannin 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose (PGG) and two simple phenolic compounds ellagic acid (EA) and gallic acid (GA) with bovine serum albumin (BSA). Fluorescence experiments showed that PGG and EA could strongly interact with BSA. The binding constants showed a pH-dependent binding of phenolic acids by BSA. The CD spectra revealed that GA, EA and PGG has slight effect on the secondary structure of BSA at the low concentration (50 μM). However, obvious alterations of the secondary structure of BSA were observed at high concentrations (from 100 μM to 500 μM). The interaction of GA, EA and PGG at high concentrations with BSA caused an unfolding of the secondary structure of BSA. PGG has a greater impact on the secondary structure of BSA when compared with small molecule compounds GA and EA.
荧光技术和圆二色性（CD）被用于研究一种典型的没食子单宁 1,2,3,4,6-五-O-没食子酰基-β-D-吡喃葡萄糖（PGG）以及两种简单的酚类化合物鞣花酸（EA）和没食子酸（GA）与牛血清白蛋白（BSA）的相互作用。荧光实验表明 PGG 和 EA 可以与 BSA 发生强烈相互作用。结合常数表明，BSA 对酚酸的结合具有 pH 依赖性。CD 光谱表明，GA、EA 和 PGG 在低浓度（50 μM）时对 BSA 的二级结构有轻微影响。然而，在高浓度（从 100 μM 到 500 μM）时，BSA 的二级结构发生了明显的变化。GA、EA 和 PGG 在高浓度时与 BSA 的相互作用导致 BSA 二级结构的展开。与小分子化合物 GA 和 EA 相比，PGG 对 BSA 二级结构的影响更大。
Zotero 插件