Dipartimento di Chimica, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70126 Bari, Italy.
Centro Interdipartimentale SMART, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70126 Bari, Italy; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari Aldo Moro, via Orabona 4, 70126 Bari, Italy.
Food Chem. 2020 Sep 15;324:126878. doi: 10.1016/j.foodchem.2020.126878. Epub 2020 Apr 21.
Ceramides (Cer) and cerebrosides are important sphingolipids (SL) involved in many biological processes. Herein, the SL content of yellow lupin seeds (Lupinus luteus) was determined by liquid-liquid extraction, mild alkaline hydrolysis (1 h at 37 °C) and reversed-phase liquid chromatography with negative electrospray ionization coupled to either an orbital-trap (Fourier-transformed, FT) or linear ion-trap (LIT) mass spectrometry (RPLC-ESI/MS). The chemical identity of SL including the sugar residues and sphingoid backbone (SB) was inferred by collision-induced dissociation multiple-stage mass spectrometry (MS, n = 2,3). Up to 52 Cer and 47 cerebrosides were successfully recognized and quantified in sample extracts of L. luteus seeds also counting isobaric species. As reported for other vegetables, a hydroxylated SB was observed whereby the N-acyl chains showed a high occurrence of very-long-chain moieties; phytosphingosine and 4-hydroxy-8-sphingenine were the predominant SB paired mainly with oleic acid (C18:1), hydroxylated behenic acid (C22:0;1) and hydroxylated lignoceric acid (C24:0;1).
神经酰胺（Cer）和脑苷脂是参与多种生物过程的重要神经鞘脂（SL）。本文采用液-液萃取、温和碱性水解（37°C 下 1 h）和反相液相色谱法，结合负电喷雾电离和轨道阱（FT）或线性离子阱（LIT）质谱（RPLC-ESI/MS），对黄羽扇豆种子（Lupinus luteus）中的 SL 含量进行了测定。通过碰撞诱导解离多级质谱（MS，n=2,3）推断 SL 的化学结构，包括糖残基和神经鞘氨醇骨架（SB）。在黄羽扇豆种子的样品提取物中，成功识别并定量了多达 52 种 Cer 和 47 种脑苷脂，同时也计算了等质量离子物种。与其他蔬菜一样，本文观察到了一种羟基化的 SB，其 N-酰基链中存在大量的超长链部分；植物鞘氨醇和 4-羟基-8-神经鞘氨醇是主要与油酸（C18:1）、羟基化山嵛酸（C22:0；1）和羟基化古柯酸（C24:0；1）配对的主要 SB。
