Sleight A J, Marsden C A, Palfreyman M G, Mir A K, Lovenberg W
Merrell Dow Research Institute, Strasbourg Center, France.
Eur J Pharmacol. 1988 Sep 23;154(3):255-61. doi: 10.1016/0014-2999(88)90199-9.
The effect of chronic administration of various monoamine oxidase (MAO) inhibitors on the ability of 8-hydroxy-2-(di-n-propylamino)tetralin (8-OH-DPAT) to inhibit forskolin-stimulated adenylate cyclase activity was studied. Groups of 12 rats were given either saline, (E)-beta-fluoromethylene-m-tyrosine (MDL 72394 0.25 mg/kg p.o.), clorgyline (1 mg/kg p.o.), selegiline (1 mg/kg p.o.) or tranylcypromine (5 mg/kg p.o.) once a day for 21 days. Biochemical determinations were made 72 h after the final dose. MDL 72394 and tranylcypromine produced a nonselective inhibition of MAO but clorgyline and selegiline selectively inhibited MAO A and MAO B respectively. All treatments that inhibited MAO A also increased tissue levels of 5-HT. Chronic treatment with MDL 72394, clorgyline or tranylcypromine reduced the ability of 8-OH-DPAT to inhibit forskolin-stimulated adenylate cyclase activity. These data suggest that chronic nonselective and chronic MAO A inhibition causes a down-regulation of the 5-HT1A-mediated inhibition of forskolin-stimulated adenylate cyclase activity.
研究了长期给予各种单胺氧化酶（MAO）抑制剂对8-羟基-2-（二正丙基氨基）四氢萘（8-OH-DPAT）抑制福斯高林刺激的腺苷酸环化酶活性能力的影响。将12只大鼠分为几组，每天一次给予生理盐水、（E）-β-氟亚甲基间酪氨酸（MDL 72394，0.25mg/kg口服）、氯吉兰（1mg/kg口服）、司来吉兰（1mg/kg口服）或反苯环丙胺（5mg/kg口服），持续21天。在最后一剂后72小时进行生化测定。MDL 72394和反苯环丙胺对MAO产生非选择性抑制，但氯吉兰和司来吉兰分别选择性抑制MAO A和MAO B。所有抑制MAO A的处理也增加了5-羟色胺的组织水平。用MDL 72394、氯吉兰或反苯环丙胺进行慢性处理降低了8-OH-DPAT抑制福斯高林刺激的腺苷酸环化酶活性的能力。这些数据表明，慢性非选择性和慢性MAO A抑制导致5-HT1A介导的福斯高林刺激的腺苷酸环化酶活性抑制的下调。
