LABIOM-R, Departamento de Odontotécnica, Universidade Federal Fluminense, Niteroi, Rio de Janeiro, Brasil.
J Appl Oral Sci. 2020;28:e20190499. doi: 10.1590/1678-7757-2019-0499. Epub 2020 Apr 27.
Enzymatic degradation of the hybrid layer can be accelerated by the activation of dentin metalloproteinases (MMP) during the bonding procedure. MMP inhibitors may be used to contain this process. Objective To evaluate the degree of conversion (DC%), dentin bond strength (µTBS) (immediate and after 1 year of storage in water), and nanoleakage of an experimental (EXP) and a commercial (SB) adhesive system, containing different concentrations of the MMP inhibitor GM1489: 0, 1 µM, 5 µM and 10 µM. Methodology DC% was evaluated by FT-IR spectroscopy. Dentin bond strength was evaluated by µTBS test. Half of beams were submitted to the µTBS test after 24 h and the other half, after storage for 1 year. From each tooth and storage time, 2 beams were reserved for nanoleakage testing. Data were analyzed using ANOVA and Tukey's test to compare means (α=0.05). Results All adhesive systems maintained the µTBS after 1 year of storage. Groups with higher concentrations of inhibitor (5 µM and 10 µM) showed higher µTBS values than groups without inhibitor or with 1 µM. The nanoleakage values of all groups showed no increase after 1 year of storage and values were similar for SB and EXP groups, in both storage periods. The inhibitor did not affect the DC% of the EXP groups, but the SB5 and SB10 groups showed higher DC% values than those of SB0 and SB1. Conclusions The incorporation of GM1489 in the adhesive systems had no detrimental effect on DC%. The concentrations of 5 µM GM1489 for SB and 5 µM or 10 µM for EXP provided higher μTBS than groups without GM1489, in the evaluation after 1 year of storage; whereas the concentration of inhibitor did not affect adhesive systems nanoleakage.
混合层的酶降解可以通过在粘结过程中牙本质金属蛋白酶（MMP）的激活而加速。可以使用 MMP 抑制剂来控制这个过程。目的：评估一种实验性（EXP）和一种商业（SB）粘结系统的转化率（DC％）、牙本质粘结强度（µTBS）（即刻和在水中储存 1 年后）和纳米渗漏程度，该系统含有不同浓度的 MMP 抑制剂 GM1489：0、1µM、5µM 和 10µM。方法：通过傅里叶变换红外光谱法评估 DC％。牙本质粘结强度通过 µTBS 测试进行评估。一半的梁在 24 小时后进行 µTBS 测试，另一半在储存 1 年后进行。从每颗牙齿和储存时间中，保留 2 个梁用于纳米渗漏测试。使用方差分析和 Tukey 检验来比较平均值（α=0.05）来分析数据。结果：所有粘结系统在储存 1 年后均保持 µTBS。含有更高浓度抑制剂（5µM 和 10µM）的组比不含抑制剂或含 1µM 的组显示出更高的 µTBS 值。所有组的纳米渗漏值在储存 1 年后均无增加，且在两个储存期内，SB 和 EXP 组的数值相似。抑制剂对 EXP 组的 DC％没有影响，但 SB5 和 SB10 组的 DC％值高于 SB0 和 SB1 组。结论：GM1489 掺入粘结系统对 DC％没有不利影响。对于 SB，GM1489 的浓度为 5µM，对于 EXP，GM1489 的浓度为 5µM 或 10µM，与不含 GM1489 的组相比，在储存 1 年后的评估中提供了更高的 µTBS；而抑制剂的浓度不影响粘结系统的纳米渗漏。
