Hambleton P, Strange R E
J Med Microbiol. 1977 May;10(2):151-60. doi: 10.1099/00222615-10-2-151.
When used to determine immunopurified antibacterial globulins in saline-phosphate buffer, the detection sensitivities of direct competitive and indirect immunoradiometric assays were respectively 25 and 2 ng. Normal human and rabbit sera interfered in both types of assay, markedly decreasing sensitivity and precision. Various pre-treatments of sera and modifications of reaction conditions substantially decreased interference in the competitive assay without affecting the activity of added antibody, but they had only marginal effects in the indirect assay. When serum samples taken from rabbits after vaccination with the Live Vaccine strain of Francisella tularensis were tested with the two assays and agglutination tests, newly synthesised circulating antibody was usually detected earlier by the competitive assay than by the other methods.
当用于在磷酸盐缓冲盐溶液中测定免疫纯化的抗菌球蛋白时，直接竞争免疫放射分析和间接免疫放射分析的检测灵敏度分别为25 ng和2 ng。正常人血清和兔血清在两种分析中均产生干扰，显著降低了灵敏度和精密度。血清的各种预处理和反应条件的改变在不影响添加抗体活性的情况下，大幅降低了竞争分析中的干扰，但对间接分析仅有微小影响。当用这两种分析方法和凝集试验检测接种土拉弗朗西斯菌活疫苗株后兔的血清样本时，竞争分析通常比其他方法更早检测到新合成的循环抗体。
