Olson G E, Winfrey V P
Department of Cell Biology, Vanderbilt University, Nashville, Tennessee 37232.
Gamete Res. 1988 Jul;20(3):329-42. doi: 10.1002/mrd.1120200308.
A purified head fraction was prepared from bovine epididymal spermatozoa and was utilized to identify the solubility characteristics and major polypeptide components of the postacrosomal sheath. Sperm heads extracted in nonionic-detergent-containing or high-salt-containing solutions retained an intact postacrosomal sheath, but it was readily solubilized by high pH extraction solutions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a major polypeptide of 58,000 daltons (58-kD) in the high pH extract solution. Antibodies to the 58-kD polypeptide specifically reacted with the postacrosomal segment by immunofluorescence and by electron microscopic immunohistochemistry were shown to bind the postacrosomal sheath. We conclude that this 58-kD polypeptide is a constituent of the postacrosomal sheath and that its distribution is restricted to the postacrosomal segment.
从牛附睾精子中制备了纯化的头部组分，用于鉴定顶体后鞘的溶解性特征和主要多肽成分。在含有非离子洗涤剂或高盐的溶液中提取的精子头部保留了完整的顶体后鞘，但它很容易被高pH值提取溶液溶解。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳显示，高pH值提取溶液中有一条主要的58,000道尔顿（58-kD）的多肽。针对58-kD多肽的抗体通过免疫荧光与顶体后段发生特异性反应，并且通过电子显微镜免疫组织化学显示其与顶体后鞘结合。我们得出结论，这种58-kD多肽是顶体后鞘的一个组成部分，并且其分布仅限于顶体后段。
