一种基于密度分离种子的简单、无毒方法及其在分离种子油突变体中的应用。
A simple, non-toxic method for separating seeds based on density, and its application in isolating seed oil mutants.
作者信息
Dean Gillian H, Pang Flora, Haughn George W, Kunst Ljerka
机构信息
Department of Botany University of British Columbia 6270 University Boulevard Vancouver V6T 1Z4 Canada.
出版信息
Appl Plant Sci. 2020 Apr 20;8(4):e11332. doi: 10.1002/aps3.11332. eCollection 2020 Apr.
PREMISE
Seed oil is an economically important trait in oilseed crops. A novel method was developed to isolate seeds with altered oil content.
METHODS AND RESULTS
In , seed oil content is correlated with seed density, with high-oil seeds being less dense than wild type and tending to float in solution, and low-oil seeds being denser and tending to sink. In contrast to previous methods, which used toxic chemicals and density gradient centrifugation, different concentrations of calcium chloride (CaCl) were employed to separate seeds without the need for centrifugation. The method was validated using known seed oil mutants, and 120,822 T-DNA mutagenized lines were then screened for novel seed density phenotypes.
CONCLUSIONS
A number of candidate mutants, as well as new alleles of two genes known to influence seed oil biosynthesis, were successfully isolated.
前提
种子油是油料作物中一项具有重要经济价值的性状。已开发出一种新方法来分离含油量改变的种子。
方法与结果
在[具体情境未提及]中，种子油含量与种子密度相关，高油种子比野生型种子密度小，倾向于漂浮在溶液中，而低油种子密度更大，倾向于下沉。与以往使用有毒化学物质和密度梯度离心的方法不同，采用不同浓度的氯化钙（CaCl）来分离种子，无需离心。该方法通过已知的种子油突变体进行了验证，然后对120,822个T-DNA诱变系进行筛选，以寻找新的种子密度表型。
结论
成功分离出了许多候选突变体以及两个已知影响种子油生物合成的基因的新等位基因。
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