在根瘤器官发生过程中表达的细胞分裂素生物合成基因受苜蓿 KNOX3 蛋白的直接调控。
Cytokinin biosynthesis genes expressed during nodule organogenesis are directly regulated by the KNOX3 protein in Medicago truncatula.
机构信息
Department of Genetics and Biotechnology, Saint Petersburg State University, Saint Petersburg, Russia.
Cell and Molecular Biology Department, Kosar University of Bojnord, Bojnord, North Khorasan Province, Iran.
出版信息
PLoS One. 2020 Apr 30;15(4):e0232352. doi: 10.1371/journal.pone.0232352. eCollection 2020.
Cytokinin is an important regulator of symbiotic nodule development. Recently, KNOTTED1-LIKE HOMEOBOX 3 transcription factor (TF) was shown to regulate symbiotic nodule development possibly via the activation of cytokinin biosynthesis genes. However, the direct interaction between the KNOX3 TF and its target genes has not been investigated up to date. Here, using EMSA analysis and SPR-based assay, we found that MtKNOX3 homeodomain directly binds to the regulatory sequences of the MtLOG1, MtLOG2, and MtIPT3 genes involved in nodulation in Medicago truncatula. Moreover, we showed that MtLOG2 and MtIPT3 expression patterns partially overlap with MtKNOX3 expression in developing nodules as it was shown by promoter:GUS analysis. Our data suggest that MtKNOX3 TF may directly activate the MtLOG1, MtLOG2, and MtIPT3 genes during nodulation thereby increasing cytokinin biosynthesis in developing nodules.
细胞分裂素是共生结瘤发育的重要调节剂。最近，同源异形盒 3 转录因子（TF）被证明通过激活细胞分裂素生物合成基因来调节共生结瘤发育。然而，到目前为止，还没有研究 KNOX3 TF 与其靶基因之间的直接相互作用。在这里，我们使用 EMSA 分析和基于 SPR 的测定法发现，MtKNOX3 同源域直接结合到调控序列中 MtLOG1、MtLOG2 和 MtIPT3 基因，这些基因参与了 Medicago truncatula 中的结瘤。此外，我们通过启动子：GUS 分析表明，MtLOG2 和 MtIPT3 的表达模式与发育中的结瘤中 MtKNOX3 的表达部分重叠。我们的数据表明，MtKNOX3 TF 可能在结瘤过程中直接激活 MtLOG1、MtLOG2 和 MtIPT3 基因，从而增加发育中的结瘤中的细胞分裂素生物合成。
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