Department of Energy Science, Alagappa University, Karaikudi 630 003, Tamil Nadu, India; Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
Bioenergy and Bioremediation Laboratory, Department of Microbiology, Alagappa University, Karaikudi 630003, Tamil Nadu, India.
Bioresour Technol. 2020 Aug;310:123447. doi: 10.1016/j.biortech.2020.123447. Epub 2020 Apr 25.
In the present study, isolation and identification of hydrogen producing strains from sugar and food industry wastewater were reported. From 48 isolates in both the wastewater, initial batch studies led to the use of four effective strains, which were identified using 16S rRNA gene sequencing as Bacillus thuringiensis-FH1, Comamonas testosteroni-FB1, Klebsiella pneumoniae-FA2 and Bacillus cereus-SB2, respectively. Further optimization studies were done at various pH values (5-8) and wastewater concentrations (10-100%). In the optimized batch experimentation, K. pneumoniae-FA2 excelled with the maximum cumulative hydrogen production of 880.93 ± 44.0 mL/L. A 3 L bioreactor was employed for effective hydrogen production, which conferred that K. pneumoniae-FA2, surpassed the other three with the maximum hydrogen yield of 3.79 ± 0.04 mol H/mol glucose. Bioelectricity production by K. pneumoniae-FA2 was also investigated in the microbial fuel cell at the optimized conditions to demonstrate its versatility in energy applications.
在本研究中，报告了从糖和食品工业废水中分离和鉴定产氢菌株的情况。从两种废水中的 48 个分离物中，初步分批研究导致使用了四种有效菌株，这些菌株通过 16S rRNA 基因测序鉴定为苏云金芽孢杆菌-FH1、粪产碱杆菌-FB1、肺炎克雷伯菌-FA2 和蜡样芽孢杆菌-SB2。在不同的 pH 值（5-8）和废水浓度（10-100%）下进行了进一步的优化研究。在优化的分批实验中，肺炎克雷伯菌-FA2 的最大累计产氢量为 880.93±44.0 mL/L。采用 3 L 生物反应器进行有效的氢气生产，结果表明，肺炎克雷伯菌-FA2 的最大氢气产率为 3.79±0.04 mol H/mol 葡萄糖，优于其他三种菌株。还在优化条件下的微生物燃料电池中研究了肺炎克雷伯菌-FA2 的生物电能产生情况，以证明其在能源应用中的多功能性。
