Groopman J D, Donahue K F
Boston University School of Public Health, Environmental Health Section, MA 02118.
J Assoc Off Anal Chem. 1988 Sep-Oct;71(5):861-7.
We have used monoclonal antibody technology to produce antibodies that recognize aflatoxins in order to develop noninvasive methods in conjunction with other chemical analytical techniques to monitor human exposure to environmental carcinogens. These methods require the ability to quantitate aflatoxins and their metabolites, including DNA and protein adducts, in readily accessible compartments such as serum and urine. The techniques permit efficient analysis of many samples in a relatively short time. Also, these monoclonal antibody affinity columns have been extremely useful for rapid isolation of aflatoxins from food and grain samples, as well as aflatoxin M1 from milk. Monoclonal antibody affinity methods are nondestructive to the aflatoxin molecule, so the sample aliquot can be used for confirmation. The use of monoclonal antibody preparative affinity columns represents a major, substantive breakthrough for analytical chemists and will be a generally applicable technology for isolation of many different substances.
我们利用单克隆抗体技术生产出能识别黄曲霉毒素的抗体，以便结合其他化学分析技术开发非侵入性方法，用于监测人体对环境致癌物的接触情况。这些方法需要具备对黄曲霉毒素及其代谢物（包括DNA和蛋白质加合物）进行定量分析的能力，且分析对象为血清和尿液等易于获取的样本。这些技术能够在相对较短的时间内对大量样本进行高效分析。此外，这些单克隆抗体亲和柱对于从食品和谷物样本中快速分离黄曲霉毒素以及从牛奶中分离黄曲霉毒素M1极为有用。单克隆抗体亲和方法对黄曲霉毒素分子无损，因此可将样本等分试样用于确证。单克隆抗体制备亲和柱的应用对分析化学家而言是一项重大的实质性突破，并且将成为分离许多不同物质的通用技术。
