Uchida A, Ebata S, Wada K, Matsubara H, Ishida Y
Department of Fisheries, Faculty of Agriculture, Kyoto University.
J Biochem. 1988 Nov;104(5):700-5. doi: 10.1093/oxfordjournals.jbchem.a122536.
The amino acid sequence of the major ferredoxin component isolated from a dinoflagellate, Peridinium bipes, was completely determined. Staphylococcus aureus V8 proteolytic, tryptic and chymotryptic peptides of Cm-ferredoxin were prepared and sequenced. The sequence was Phe-Lys-Val-Thr-Leu-Asp-Thr-Pro-Asp-Gly-Lys-Lys-Ser-Phe-Glu-Cys- Pro-Gly-Asp-Ser-Tyr-Ile-Leu-Asp-Lys-Ala-Glu-Glu-Glu-Gly-Leu-Glu-Leu-Pro- Tyr-Ser - Cys-Arg-Ala-Gly-Ser-Cys-Ser-Ser-Cys-Ala-Gly-Lys-Val-Leu-Thr-Gly-Ser-Ile- Asp-Gln - Ser-Asp-Gln-Ala-Phe-Leu-Asp-Asp-Asp-Gln-Gly-Gly-Asp-Gly-Tyr-Cys-Leu-Thr- Cys-Val - Thr-Tyr-Pro-Thr-Ser-Asp-Val-Thr-Ile-Lys-Thr-His-Cys-Glu-Ser-Glu-Leu. It was composed of 93 amino acid residues with 7 cysteine residues, the highest number found among the chloroplast-type ferredoxins so far sequenced. A cysteine residue was found for the first time at the 89th position in a chloroplast-type ferredoxin. Calculation of the numbers of amino acid differences among chloroplast-type ferredoxins indicates that the Peridinium ferredoxin is far divergent not only from higher plant ferredoxins but also from blue-green algal ferredoxins.
从双鞭甲藻（Peridinium bipes）中分离出的主要铁氧化还原蛋白成分的氨基酸序列已完全确定。制备了嗜热栖热放线菌V8蛋白酶、胰蛋白酶和胰凝乳蛋白酶作用于Cm-铁氧化还原蛋白产生的肽段并进行了测序。序列为：苯丙氨酸-赖氨酸-缬氨酸-苏氨酸-亮氨酸-天冬氨酸-苏氨酸-脯氨酸-天冬氨酸-甘氨酸-赖氨酸-赖氨酸-丝氨酸-苯丙氨酸-谷氨酸-半胱氨酸-脯氨酸-甘氨酸-天冬氨酸-丝氨酸-酪氨酸-异亮氨酸-亮氨酸-天冬氨酸-赖氨酸-丙氨酸-谷氨酸-谷氨酸-谷氨酸-甘氨酸-亮氨酸-谷氨酸-亮氨酸-脯氨酸-酪氨酸-丝氨酸-半胱氨酸-精氨酸-丙氨酸-甘氨酸-丝氨酸-半胱氨酸-丝氨酸-丝氨酸-半胱氨酸-丙氨酸-甘氨酸-赖氨酸-缬氨酸-亮氨酸-苏氨酸-甘氨酸-丝氨酸-异亮氨酸-天冬氨酸-谷氨酰胺-丝氨酸-天冬氨酸-谷氨酰胺-丙氨酸-苯丙氨酸-亮氨酸-天冬氨酸-天冬氨酸-天冬氨酸-谷氨酰胺-甘氨酸-甘氨酸-天冬氨酸-甘氨酸-酪氨酸-半胱氨酸-亮氨酸-苏氨酸-半胱氨酸-缬氨酸-苏氨酸-酪氨酸-脯氨酸-苏氨酸-丝氨酸-天冬氨酸-缬氨酸-苏氨酸-异亮氨酸-赖氨酸-苏氨酸-组氨酸-半胱氨酸-谷氨酸-丝氨酸-谷氨酸-亮氨酸。它由93个氨基酸残基组成，含有7个半胱氨酸残基，这是迄今为止已测序的叶绿体型铁氧化还原蛋白中半胱氨酸残基数量最多的。在叶绿体型铁氧化还原蛋白的第89位首次发现了一个半胱氨酸残基。叶绿体型铁氧化还原蛋白之间氨基酸差异数的计算表明，双鞭甲藻铁氧化还原蛋白不仅与高等植物铁氧化还原蛋白差异很大，而且与蓝藻铁氧化还原蛋白也有很大差异。
