Cold Spring Harb Protoc. 2020 Jun 1;2020(6):102152. doi: 10.1101/pdb.prot102152.
The general strategy of the baculovirus expression system is to infect insect cells with a virus that expresses a foreign protein at a very late stage of infection. Almost all baculovirus expression systems use the procedures for insect cell transfection, baculovirus production, and protein expression given in the main portion of this protocol. This protocol also includes a method that uses molecular biology techniques to produce recombinant baculovirus DNA in before transfection of insect cells. It is important to quantify the viral titer to achieve optimal and reproducible expression of target proteins. Accordingly, the viral plaque assay is also described here.
杆状病毒表达系统的一般策略是，用一种在感染后期表达外源蛋白的病毒感染昆虫细胞。几乎所有的杆状病毒表达系统都使用本方案主要部分中给出的昆虫细胞转染、杆状病毒生产和蛋白表达程序。本方案还包括一种使用分子生物学技术在转染昆虫细胞之前产生重组杆状病毒 DNA 的方法。定量病毒效价对于实现目标蛋白的最佳和可重复表达非常重要。因此，这里也描述了病毒空斑测定法。
