肉毒杆菌神经毒素A、B和E型的快速蛋白质液相色谱法

Fast protein liquid chromatography of botulinum neurotoxin types A, B and E.

作者信息

Woody M A, DasGupta B R

机构信息

Food Research Institute, University of Wisconsin, Madison 53706.

出版信息

J Chromatogr. 1988 Sep 9;430(2):279-89. doi: 10.1016/s0378-4347(00)83163-1.

DOI:10.1016/s0378-4347(00)83163-1

PMID:3235504

Abstract

Three antigenically different botulinum neurotoxins (NTs, relative molecular mass approximately 150,000), classically distinguished only by specific antisera, were for the first time chromatographically resolved. Mixed NTs eluted from a Mono-Q column in order of types E, A and B, and from Mono-S as B, E and A. Type A and B NTs were successfully chromatographed on the cation-exchange Mono-S column above their isoelectric points. Purification of type A and B NTs by automated liquid chromatography was also accomplished for the first time. Type A, B and E NTs were purified by application on an anion-exchange Mono-Q column, followed by use of a cation-exchange Mono-S column.

摘要

三种抗原性不同的肉毒杆菌神经毒素（NTs，相对分子质量约为150,000），传统上仅通过特异性抗血清区分，首次通过色谱法分离出来。混合的NTs从Mono-Q柱上按E型、A型和B型的顺序洗脱，从Mono-S柱上按B型、E型和A型的顺序洗脱。A型和B型NTs在高于其等电点的条件下成功地在阳离子交换Mono-S柱上进行了色谱分离。A型和B型NTs的自动液相色谱纯化也首次得以实现。通过应用阴离子交换Mono-Q柱，随后使用阳离子交换Mono-S柱，对A型、B型和E型NTs进行了纯化。