[B型肉毒杆菌神经毒素的均质化生产]

[Production of a homogeneous Cl. botulinum type B neurotoxin].

作者信息

Saprykina T P, Kliucheva V V, Blagoveshchenskiĭ V A, Mironova M V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1980 Sep(9):86-91.

Abstract

The method for obtaining the neurotoxin, or alpha-fraction of the toxin, of Cl. botulinum, type B, is described. In accordance with this method, the toxin was precipitated three times with hydrochloric acid in the isoelectric zone with subsequent extraction with phosphate (pH 6.8) and citrate-phosphate (pH 5.6) buffers, then fractionated in columns with DEAE cellulose (pH 5.6), DEAE Sephadex A-50 (pH 7.2) and Sephadex G-200 (pH 7.2). The homogeneous neurotoxin preparations with molecular weights ranging from 145,000 to 160,000 and having the isoelectric point at pH 5.5 and toxicity 5.0--10.0 x 10(7) Dlm per 1 mg protein were obtained.

摘要

描述了获取B型肉毒梭菌神经毒素或毒素α组分的方法。按照该方法，毒素在等电区用盐酸沉淀三次，随后用磷酸盐（pH 6.8）和柠檬酸-磷酸盐（pH 5.6）缓冲液萃取，然后在装有DEAE纤维素（pH 5.6）、DEAE葡聚糖A-50（pH 7.2）和葡聚糖G-200（pH 7.2）的柱中进行分级分离。得到了分子量在145,000至160,000之间、等电点为pH 5.5且毒性为每1毫克蛋白质5.0--10.0×10(7) Dlm的均一神经毒素制剂。