Khachik F, Beecher G R
U.S. Department of Agriculture, Beltsville Human Nutrition Research Center, MD 20705.
J Chromatogr. 1988 Sep 30;449(1):119-33. doi: 10.1016/s0021-9673(00)94373-5.
Employing isocratic and gradient-elution high-performance liquid chromatography (HPLC) a number of straight-chain fatty acid esters (decanoate, laurate, myristate, palmitate) of violaxanthin, auroxanthin, lutein, zeaxanthin, isozeaxanthin, and beta-cryptoxanthin, prepared by partial synthesis, have been separated on a C18 reversed-phase column. Several chromatographic conditions were developed that separated a mixture of di-fatty acid esters (dimyristate, myristate palmitate mixed ester, dipalmitate) of violaxanthin, auroxanthin, lutein, and zeaxanthin in a single chromatographic run. Hydroxycarotenoids such as lutein, zeaxanthin, and isozeaxanthin that are not easily separated by HPLC on C18 reversed-phase columns, can be readily separated after derivatization with fatty acids and chromatography of their esters. Chromatographic conditions for optimum separation of carotenoids from various classes are discussed.
采用等度洗脱和梯度洗脱高效液相色谱法（HPLC），在C18反相柱上分离了通过部分合成制备的紫黄质、金盏花黄质、叶黄素、玉米黄质、异玉米黄质和β-隐黄质的多种直链脂肪酸酯（癸酸酯、月桂酸酯、肉豆蔻酸酯、棕榈酸酯）。开发了几种色谱条件，可在一次色谱运行中分离紫黄质、金盏花黄质、叶黄素和玉米黄质的二脂肪酸酯混合物（二肉豆蔻酸酯、肉豆蔻酸棕榈酸混合酯、二棕榈酸酯）。在C18反相柱上不易通过HPLC分离的羟基类胡萝卜素，如叶黄素、玉米黄质和异玉米黄质，在用脂肪酸衍生化并对其酯进行色谱分析后可轻松分离。讨论了从各类中最佳分离类胡萝卜素的色谱条件。
