College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
Mikrochim Acta. 2020 Apr 30;187(5):306. doi: 10.1007/s00604-020-04255-1.
A strip test is described for the optical determination of influenza virus H3 subtype. It utilizes gold nanoparticle (AuNP) coated polystyrene latex microspheres (PS) as the label and a sandwich format. The AuNP and PS particles were linked using monoclonal antibodies against influenza virus as the bridge. Under the optimal conditions, the visual detection limit of the AuNP-PS-based strip test was as low as 1/16 hemagglutination unit (HAU). It was 64 times higher than that of 10 nm (4 HAU) AuNP-based strip tests. Quantitative analysis showed that the detection limit of the AuNP-PS-based strip is 0.016 HAU. The AuNP-PS-based strip test showed no cross-reactivity to the other subtypes (H1, H5, H7, or H9) of influenza viruses. Graphical abstract .
一种用于光学测定流感病毒 H3 亚型的条带检测方法。它利用金纳米颗粒（AuNP）包覆的聚苯乙烯乳胶微球（PS）作为标记物，并采用三明治结构。AuNP 和 PS 颗粒通过针对流感病毒的单克隆抗体连接作为桥梁。在最佳条件下，基于 AuNP-PS 的条带检测的目视检测限低至 1/16 血凝单位（HAU）。比基于 10nm（4HAU）AuNP 的条带检测高 64 倍。定量分析表明，基于 AuNP-PS 的条带检测的检测限为 0.016HAU。基于 AuNP-PS 的条带检测与其他流感病毒亚型（H1、H5、H7 或 H9）没有交叉反应。
