Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, University of Campania "Luigi Vanvitelli", Naples, Italy.
Electrophoresis. 2020 Oct;41(20):1740-1748. doi: 10.1002/elps.202000028. Epub 2020 May 18.
Chondroitin sulfate is extracted from animal cartilaginous tissues and is commercialized as active principle against osteoarthritis. Its biological activity depends on its purity grade and could be altered by the presence of other glycosaminoglycans like keratan sulfate that could be contemporarily extracted from animal tissues or like hyaluronic acid that, instead, is added on purpose in food supplements. Although numerous methods are reported in literature for quality control analyses of chondroitin sulfate, few of them are able to detect other glycosaminoglycans. In this paper, for the first time, a new high-performance CE method was set up to quantify the chondroitin sulfate, the eventual keratan sulfate, and hyaluronic acid as intact chains: five chondroitin sulfate standards and 13 animal origin samples or food supplements from six different suppliers were analyzed. The new method was able to determine keratan sulfate similarly to a previously reported high-performance anion-exchange chromatography method, but in addition it showed the advantage to determine also the hyaluronic acid as never reported before.
硫酸软骨素从动物软骨组织中提取，作为治疗骨关节炎的有效成分商业化。其生物活性取决于其纯度等级，可能会因同时提取自动物组织的其他糖胺聚糖（如硫酸角质素）或故意添加到食品补充剂中的透明质酸而发生改变。尽管文献中报道了许多硫酸软骨素质量控制分析方法，但很少有方法能够检测到其他糖胺聚糖。在本文中，首次建立了一种新的高效 CE 方法，以定量分析完整链形式的硫酸软骨素、可能存在的硫酸角质素和透明质酸：分析了 5 种硫酸软骨素标准品和 13 种来自 6 个不同供应商的动物来源样品或食品补充剂。与之前报道的高效阴离子交换色谱法类似，新方法能够测定硫酸角质素，但与之前的方法相比，它还有一个优势，就是能够测定以前从未报道过的透明质酸。
