Osamura R Yoshiyuki, Yasuda O, Kawai K, Hori S, Suemizu H, Onoda N, Joh Tong H
Department of Pathology, Division of Diagnostic Pathology, Tokai University School of Medicine, Boseidai Isehara-City 259-11, Kanagawa, Japan.
Endocr Pathol. 1990 Jun;1(2):102-108. doi: 10.1007/BF02915625.
A total of 17 (10 adrenal and 7 extraadrenal) pheochromocytomas were examined immunohistochemically for the localization of catecholamine-synthesizing enzymes-tyrosine hydroxylase (TH), dopamine β-hydroxylase (DBH), and phenylethanolamine N-methyltransferase (PNMT)-as well as the marker peptides for pheochrornocytomas (i.e., met-enkephalin-arggly-leu [MEAGL]). Normal adult, fetal, and newborn human adrenal medullas were also examined. Six adrenal pheochromocytomas showed immunohistochernically positive staining for PNMT; 5 of these cases demonstrated elevated serum adrenalin levels, This indicated morphofunctional correlation. PNMT-positive cells were mostly positive for TH but did not show co-localization of MEAGL except in 1 case, Absence of co-localization was considered to reflect the physiological condition of the specimen, based on the similar staining in the normal adult adrenal medulla. Primary culture and immunoelectron microscopy suggested the processing and synthesis of adrenalin and MEAGL in the secretory granules.Seven extraadrenal pheochromocytornas were negative for PNMT and suggested the lack of adrenalin synthesis.
对总共17例嗜铬细胞瘤（10例肾上腺嗜铬细胞瘤和7例肾上腺外嗜铬细胞瘤）进行了免疫组织化学检查，以确定儿茶酚胺合成酶（酪氨酸羟化酶[TH]、多巴胺β羟化酶[DBH]和苯乙醇胺N甲基转移酶[PNMT]）的定位，以及嗜铬细胞瘤的标记肽（即甲硫氨酸脑啡肽-精氨酸-甘氨酸-亮氨酸[MEAGL]）的定位。还检查了正常成人、胎儿和新生儿的肾上腺髓质。6例肾上腺嗜铬细胞瘤免疫组织化学检查显示PNMT染色阳性；其中5例血清肾上腺素水平升高，这表明存在形态功能相关性。PNMT阳性细胞大多TH染色阳性，但除1例病例外，未显示MEAGL的共定位。基于正常成人肾上腺髓质中相似的染色情况，共定位的缺失被认为反映了标本的生理状态。原代培养和免疫电子显微镜检查提示肾上腺素和MEAGL在分泌颗粒中进行加工和合成。7例肾上腺外嗜铬细胞瘤PNMT染色阴性，提示缺乏肾上腺素合成。
