Rutanen Eeva-Marja, Gonzalez Elisa, Said Jonathan, Braunstein Glenn D
Department of Obstetrics and Gynecology, Minerva Institute for Medical Research, Helsinki, Finland.
Departments of Medicine, UCLA Medical Center, University of California School of Medicine, Los Angeles, CA.
Endocr Pathol. 1991 Sep;2(3):132-138. doi: 10.1007/BF02915453.
Human insulinlike growth factor binding protein-1 (hlGFBP-1) is a secretory protein that modulates the receptor-binding and biological actions of the insulinlike growth factor I (IGF-I). Human endometrium expresses the mRNA for IGFBP-1, and this protein is secreted by the secretory phase and pregnancy endometrium as well as by ovarian granulosa cells in vitro. In this study, we examined the cellular localization of IGFBP-1 in female reproductive tissues by using a purified monoclonal antibody Mab 6303 with an immunoperoxidase technique. Proliferative- and early secretory-phase endometrium as well as all extrauterine tissues except decidualized cells at the implantation site on the ovaries of ovarian pregnancies were negative for IGFBP-1. In midsecretory-phase endometrium, focal staining was first observed in the cytoplasm of glandular epithelial cells, with weaker staining in the stromal cells. In late secretoryphase endometrium, strong immunostaining was observed in predecidualized stromal cells, with weak focal staining remaining in some of the glandular epithelial cells. In early pregnancy, intense staining was detected in the cytoplasm of decidualized stromal cells of zona compacta in each sample, whereas the nondecidualized stromal cells remained unstained. Strong to medium staining was detected simultaneously in the glandular epithelial cells in 70% of the early pregnancy specimens. In term pregnancy, IGFBP-1 was localized in decidual cells of placental bed and decidua parietalis. Immunolocalization of IGFBP-1 to both endometrial epithelial and stromal cells, although only stromal cells express the gene of IGFBP-1 [14], supports the hypothesis of paracrine actions between these cells. The localization of IGFBP-1 to decidualized cells at the extrauterine implantation sites implies its association with decidual differentiation.
人胰岛素样生长因子结合蛋白-1（hlGFBP-1）是一种分泌蛋白，可调节胰岛素样生长因子I（IGF-I）的受体结合和生物学作用。人子宫内膜表达IGFBP-1的mRNA，该蛋白在分泌期和妊娠子宫内膜以及体外卵巢颗粒细胞中分泌。在本研究中，我们使用纯化的单克隆抗体Mab 6303和免疫过氧化物酶技术检测了IGFBP-1在女性生殖组织中的细胞定位。增殖期和早分泌期子宫内膜以及除卵巢妊娠卵巢植入部位蜕膜化细胞外的所有子宫外组织，IGFBP-1均为阴性。在分泌中期子宫内膜中，首先在腺上皮细胞的细胞质中观察到局灶性染色，基质细胞中的染色较弱。在分泌晚期子宫内膜中，在前蜕膜化基质细胞中观察到强免疫染色，一些腺上皮细胞中仍有弱局灶性染色。在妊娠早期，每个样本致密带蜕膜化基质细胞的细胞质中检测到强烈染色，而非蜕膜化基质细胞仍未染色。在70%的妊娠早期标本中，腺上皮细胞同时检测到强至中度染色。在足月妊娠时，IGFBP-1定位于胎盘床和子宫壁蜕膜的蜕膜细胞中。IGFBP-1在子宫内膜上皮细胞和基质细胞中的免疫定位，尽管只有基质细胞表达IGFBP-1的基因[14]，支持了这些细胞之间旁分泌作用的假说。IGFBP-1在子宫外植入部位蜕膜化细胞中的定位意味着它与蜕膜分化有关。
