Fudem-Goldin B, Voulalas P, Orr G A
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, NY 10461.
J Biochem Biophys Methods. 1988 Nov;17(3):199-202. doi: 10.1016/0165-022x(88)90004-8.
UDP[6-3H]galactose of high specificity can be obtained by oxidation of the C-6 hydroxymethyl group of UDP-galactose by galactose oxidase and subsequent reduction by sodium borotritide. One-step purification of the nucleotide sugar involves anion-exchange chromatography on a Pharmacia Mono Q column. Radiolabeled UDP-N-acetylgalactosamine can also be synthesized and purified by this procedure. Both nucleotide sugars can be used for sugar incorporation studies using the appropriate glycosyltransferase.
高特异性的UDP[6-³H]半乳糖可通过用半乳糖氧化酶氧化UDP-半乳糖的C-6羟甲基，随后用硼氢化三钠还原得到。核苷酸糖的一步纯化涉及在Pharmacia Mono Q柱上进行阴离子交换色谱。放射性标记的UDP-N-乙酰半乳糖胺也可通过此方法合成和纯化。两种核苷酸糖均可用于使用适当糖基转移酶的糖掺入研究。
