Yoneyama K
Department of Obstetrics and Gynecology, Nippon Medical School, Tokyo.
Nihon Sanka Fujinka Gakkai Zasshi. 1988 Nov;40(11):1740-6.
Differentiation and proliferation of the alveolar epithelial cells in fetal rabbit lung were studied by using immunohistochemical techniques. To observe the differentiation of type II alveolar epithelial cells, an antibody to purified rabbit surfactant was prepared and its specificity was checked by Western blot analysis. This antibody reacted with the 29-35 kDa protein group, which was presumed to be the major surfactant associated protein group in rabbit. After immunohistochemical staining, surfactant stained type II cells first appeared during the canalicular stage of fetal lung development. And they were detected prominently in the proximal portion of primitive alveolar zones and the number of stained cells increased rapidly in the succeeding gestational days. Clara cells were also reacted with this antibody during the alveolar stage. The distribution of DNA-synthesizing cells in the developing lung indicated that proliferation of alveolar epithelial cells is reduced earlier in the proximal portion than in the distal portion of primitive alveolar zones. These results suggest that differentiation of type II cells begins during the canalicular stage and it begins earlier in the proximal portion of primitive alveolar zones, which has already ceased prominent proliferation.
采用免疫组织化学技术研究了胎兔肺中肺泡上皮细胞的分化和增殖。为观察Ⅱ型肺泡上皮细胞的分化，制备了抗纯化兔表面活性剂的抗体，并通过蛋白质免疫印迹分析检测其特异性。该抗体与29 - 35 kDa蛋白组发生反应，推测该蛋白组为兔主要的表面活性剂相关蛋白组。免疫组织化学染色后，表面活性剂染色的Ⅱ型细胞在胎儿肺发育的小管期首次出现。它们在原始肺泡区的近端显著检测到，且在随后的妊娠天数中染色细胞数量迅速增加。在肺泡期，克拉拉细胞也与该抗体发生反应。发育肺中DNA合成细胞的分布表明，原始肺泡区近端肺泡上皮细胞的增殖比远端更早减少。这些结果表明，Ⅱ型细胞的分化始于小管期，且在原始肺泡区近端更早开始，而该区域已经停止显著增殖。
