Key Laboratory of Crop Growth and Development Regulation of Jiangxi Province, College of Life Sciences and Resource Environment, Yichun University, Yichun, China.
State Key Laboratory of Rice Biology & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.
Mol Ecol Resour. 2020 Jul;20(4):1050-1068. doi: 10.1111/1755-0998.13182. Epub 2020 Jun 7.
The fall armyworm (Spodoptera frugiperda) is a lepidopteran insect pest that causes huge economic losses. This notorious insect pest has rapidly spread over the world in the past few years. However, the mechanisms of rapid dispersal are not well understood. Here, we report a chromosome-level assembled genome of the fall armyworm, named the ZJ-version, using PacBio and Hi-C technology. The sequenced individual was a female collected from the Zhejiang province of China and had high heterozygosity. The assembled genome size of ZJ-version was 486 Mb, containing 361 contigs with an N50 of 1.13 Mb. Hi-C scaffolding further assembled the genome into 31 chromosomes and a portion of W chromosome, representing 97.4% of all contigs and resulted in a chromosome-level genome with scaffold N50 of 16.3 Mb. The sex chromosomes were identified by genome resequencing of a single male pupa and a single female pupa. About 28% of the genome was annotated as repeat sequences, and 22,623 protein-coding genes were identified. Comparative genomics revealed the expansion of the detoxification-associated gene families, chemoreception-associated gene families, nutrition metabolism and transport system gene families in the fall armyworm. Transcriptomic and phylogenetic analyses focused on these gene families revealed the potential roles of the genes in polyphagia and invasion of fall armyworm. The high-quality of the fall armyworm genome provides an important genomic resource for further explorations of the mechanisms of polyphagia and insecticide resistance, as well as for pest management of fall armyworm.
草地贪夜蛾（Spodoptera frugiperda）是一种鳞翅目昆虫害虫，会造成巨大的经济损失。这种声名狼藉的害虫在过去几年中迅速在世界各地蔓延。然而，其快速扩散的机制还不是很清楚。在这里，我们利用 PacBio 和 Hi-C 技术报道了草地贪夜蛾的一个染色体水平组装基因组，命名为 ZJ 版。测序个体是从中国浙江省采集的一只雌性，具有很高的杂合度。ZJ 版的组装基因组大小为 486 Mb，包含 361 个 contigs，N50 为 1.13 Mb。Hi-C 支架进一步将基因组组装成 31 条染色体和一部分 W 染色体，代表所有 contigs 的 97.4%，并生成了染色体水平的基因组，支架 N50 为 16.3 Mb。通过对单个雄性蛹和单个雌性蛹的基因组重测序鉴定了性染色体。大约 28%的基因组被注释为重复序列，鉴定出 22623 个蛋白质编码基因。比较基因组学揭示了草地贪夜蛾解毒相关基因家族、化学感受相关基因家族、营养代谢和运输系统基因家族的扩张。对这些基因家族的转录组和系统发育分析揭示了基因在暴食和草地贪夜蛾入侵中的潜在作用。高质量的草地贪夜蛾基因组为进一步探索暴食和杀虫剂抗性的机制以及草地贪夜蛾的害虫管理提供了重要的基因组资源。
