Fedorova O S, Podust L M
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
J Inorg Biochem. 1988 Nov;34(3):149-55. doi: 10.1016/0162-0134(88)85025-6.
It was shown that the treatment of a DNA fragment and a hepta-decanucleotide having a hairpin structure by Ru(2,2'-bipyridyl)3(3+) complex at 15 degrees C in 1 M NaCl for 10-60 min, and subsequent hydrolysis in 1 M piperidine at 95 degrees C led to the specific cleavage of the polynucleotide chain at guanosine residues in the single-stranded regions. This method can be used for the studying of nucleic acids secondary structure.
结果表明，在15℃下于1M氯化钠中用Ru(2,2'-联吡啶)3(3+)配合物处理具有发夹结构的DNA片段和十七聚核苷酸10 - 60分钟，随后在95℃下于1M哌啶中水解，会导致多核苷酸链在单链区域的鸟苷残基处发生特异性切割。该方法可用于研究核酸的二级结构。
