Simultaneous nanoencapsulation of lipoic acid and resveratrol with improved antioxidant properties for the skin.

Cutaneous aging is intimately related to redox imbalance, which is mainly caused by ultraviolet radiation exposure. The aim of the present investigation was to develop lipid-core nanocapsules for the co-nanoencapsulation of resveratrol and lipoic acid aiming to improve the chemical stability and photostability of the compounds, as well as their antioxidant properties. Lipid-core nanocapsules were developed and characterized according to their mean size, size distribution, zeta potential, pH value, drug content, encapsulation efficiency, release profile, stability under storage, photostability and skin permeation profile. In vitro antioxidant activity was analyzed by lipid peroxidation method and the in vitro cytotoxicity by MTT assay and cellular count, using BALB/c-3T3 fibroblasts. It was possible to co-nanoencapsulate resveratrol and lipoic acid into particles of average diameter close to 200 nm, low polydispersity index and encapsulation efficiencies around 90 %. Nanoencapsulation increased the substances stability under storage and photostability under UVA light exposure, besides controlling substances release. The actives were able to permeate a skin model membrane when nanoencapsulated, with a faster permeation of lipoic acid. The antioxidant activity was potentiated by the co-nanoencapsulation of resveratrol and lipoic acid, without signs of cytotoxicity to fibroblasts. Therefore, the co-nanoencapsulation of resveratrol and lipoic acid is promising for application in topical formulations aiming antioxidant effects.