Storm G, Steerenberg P A, van Borssum Waalkes M, Emmen F, Crommelin D J
Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, The Netherlands.
J Pharm Sci. 1988 Oct;77(10):823-30. doi: 10.1002/jps.2600771002.
This paper addresses several potential problems which may play a critical role in the outcome of in vitro studies designed to investigate the antitumor activity of drugs. These problems were demonstrated to exist in in vitro assays developed for the evaluation of antitumor activity of free and liposome-entrapped doxorubicin (DXR). The stability of DXR-containing liposomes against drug leakage into the culture medium, as well as the chemical stability and extent of adsorption to tissue culture plastics of both free and liposomal DXR during the liposome-tumor cell incubation, were investigated. It is concluded that a full understanding of these processes is required for a reliable interpretation of the experimental results.
本文探讨了几个可能对旨在研究药物抗肿瘤活性的体外研究结果起关键作用的潜在问题。这些问题在为评估游离和脂质体包裹的阿霉素（DXR）的抗肿瘤活性而开发的体外试验中得到了证实。研究了含DXR的脂质体对药物泄漏到培养基中的稳定性，以及在脂质体-肿瘤细胞孵育过程中游离和脂质体DXR的化学稳定性和对组织培养塑料的吸附程度。得出的结论是，要可靠地解释实验结果，需要全面了解这些过程。
