Nakamura Masato, Kamehama Tomoaki, Sato Yasushi
Graduate School of Science and Engineering, Ehime University, Matsuyama, Ehime 790-8577, Japan.
Faculty of Science, Ehime University, Matsuyama, Ehime 790-8577, Japan.
Plant Biotechnol (Tokyo). 2020 Mar 25;37(1):105-109. doi: 10.5511/plantbiotechnology.20.0110a.
In vascular plants, lignin is deposited during morphogenesis but also under stress conditions. Assessing the degree of stress-induced lignin deposition is complicated because it occurs locally and irregularly in plant tissues. In this study, we developed a macro program, LigninJ, for the open-source software ImageJ to automatically and efficiently determine areas and levels of lignification after Wiesner (phloroglucinol-HCl) staining. We used the CIELAB color space for detection of red color following the Wiesner reaction. In addition, LigninJ has a function for adjusting the background level and its white balance to reduce biases that are inherent to individual color images. Furthermore, LigninJ can be used for batch analyses of multiple images, taking about 2 s per image. In this study, we analyzed wound-induced lignin deposition in cotyledons of the ecotypes Landsberg and Columbia and assessed ectopic lignin depositions in roots of () mutants of . Our results confirmed that this method is efficient for evaluating the degree of stress-induced lignin deposition.
在维管植物中，木质素在形态发生过程中沉积，也在胁迫条件下沉积。评估胁迫诱导的木质素沉积程度很复杂，因为它在植物组织中局部且不规则地发生。在本研究中，我们为开源软件ImageJ开发了一个宏程序LigninJ，用于在 Wiesner（间苯三酚 - 盐酸）染色后自动且高效地确定木质化区域和水平。我们使用CIELAB颜色空间来检测Wiesner反应后的红色。此外，LigninJ具有调整背景水平及其白平衡的功能，以减少单个彩色图像固有的偏差。此外，LigninJ可用于多个图像的批量分析，每张图像大约需要2秒。在本研究中，我们分析了生态型Landsberg和Columbia子叶中伤口诱导的木质素沉积，并评估了拟南芥（）突变体根中的异位木质素沉积。我们的结果证实，该方法对于评估胁迫诱导的木质素沉积程度是有效的。
