Jiang Kai Ren, Huang Jie-Len, Chen Chia-Chun, Su Hung-Ju, Wu Jui-Chuang
Chemical Engineering Department, Chung Yuan Christian University, Chung Li, Tao Yuan 32023, Taiwan.
Biomedical Engineering Center, Industrial Technology Research Institute, Chu Tung, Hsin Chu 31040, Taiwan.
J Taiwan Inst Chem Eng. 2011 Jan;42(1):5-12. doi: 10.1016/j.jtice.2010.04.012. Epub 2010 Oct 30.
The effect of relative size of two co-axially hybridized gene targets on the hybridization efficiency was studied for two DNA probe configurations and various probe concentrations. Each of two sets of microarrayed probes contained a pair of DNA probes and a pair of their complementary samples labeled with two distinct fluorescent dyes. The sequence of each probe is especially designed so that two targets are simultaneously complementary to two adjacent sections of the probe. The molecular steric effect on the hybridization efficiency is investigated by comparing the dye signals between configurations of one-target and two-target hybridization scenarios. The results show that a low probe concentration gives better hybridization efficiency and the first-hybridization conducted by a shorter-size DNA target improves the hybridization efficiency of the second target coupling onto the same probe.
针对两种DNA探针配置和不同探针浓度，研究了两个同轴杂交基因靶标的相对大小对杂交效率的影响。两组微阵列探针中的每一组都包含一对DNA探针以及一对用两种不同荧光染料标记的互补样本。每个探针的序列经过特殊设计，使得两个靶标同时与探针的两个相邻部分互补。通过比较单靶标和双靶标杂交情况配置之间的染料信号，研究了分子空间位阻效应对杂交效率的影响。结果表明，低探针浓度可产生更好的杂交效率，由较短大小的DNA靶标进行的首次杂交可提高第二个靶标与同一探针偶联的杂交效率。
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