[鼻咽样本的病毒学分析能否反映儿童群体的下呼吸道感染情况？一项多重聚合酶链反应研究]

[Does the nasopharyngeal samples virological analysis reflect the lower respiratory tract infection in children population? A PCR multiplex study].

作者信息

Koenig-Zores C, Stoll-Keller F, Ammouche C, Donato L

机构信息

Service de réanimation néonatale et pédiatrie 2, pôle médicochirurgical de pédiatrie, hôpital de Hautepierre, hôpitaux universitaires de Strasbourg, avenue Molière, 67098 Strasbourg, France.

Service de virologie, pôle de biologie, hôpitaux universitaires de Strasbourg, 67000 Strasbourg, France.

出版信息

Rev Fr Allergol (2009). 2013 Mar;53(2):59-64. doi: 10.1016/j.reval.2012.11.007. Epub 2012 Dec 20.

DOI:10.1016/j.reval.2012.11.007

PMID:32362956

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7185594/

Abstract

Respiratory tract infections are frequent in young children and are related to viruses in most cases. Multiplex Polymerase chain reaction (PCR) based techniques are valuable tools for describing the spectrum of such viruses. The goal of this study was to assess the correlation of virus detection in samples obtained by nasopharyngeal aspiration and by bronchoalveolar lavage. Both samples were taken at the same time in 30 children with lower respiratory tract infection, and were analyzed by multiplex virus PCR (xTAG™ RVP). A strong correlation has been found ( = 0.0002) and the most frequently isolated virus was the entero-rhinovirus spp. These results strengthen the opinion that viruses colonize both the upper and lower respiratory tract. Nasopharyngeal samples should be sufficient to the diagnosis of lower respiratory tract viral infection in immuno-competent children.

摘要

呼吸道感染在幼儿中很常见，且大多数情况下与病毒有关。基于多重聚合酶链反应（PCR）的技术是描述此类病毒谱的宝贵工具。本研究的目的是评估通过鼻咽抽吸和支气管肺泡灌洗获得的样本中病毒检测的相关性。在30名患有下呼吸道感染的儿童中，同时采集了这两种样本，并通过多重病毒PCR（xTAG™ RVP）进行分析。发现两者具有很强的相关性（P = 0.0002），最常分离出的病毒是肠道鼻病毒属。这些结果强化了病毒在上呼吸道和下呼吸道均有定植的观点。对于免疫功能正常的儿童，鼻咽样本应足以诊断下呼吸道病毒感染。