大鼠脑纹状体和下丘脑多巴胺敏感腺苷酸环化酶的比较研究。

Comparative study of the dopamine-sensitive adenylate cyclase in the striatum and hypothalamus of rat brain.

作者信息

Roufogalis B D, Thornton M, Wade D N

出版信息

J Pharm Pharmacol. 1979 Jan;31(1):33-8. doi: 10.1111/j.2042-7158.1979.tb13417.x.

DOI:10.1111/j.2042-7158.1979.tb13417.x

Abstract

Stimulation by dopamine of adenylate cyclase in homogenates of rat brain striatum was enhanced in the presence of ATP (0.6--3 mM) and GTP (10--100 micrometer). The stimulation by dopamine appeared to be the result of its antagonism of inhibition of adenylate cyclase by GTP or higher concentrations of ATP. Stimulation of the enzyme by dopamine was also dependent on MgCl2, and was maximal at MgCl2 concentrations of at least two fold excess over ATP. While ATP did not inhibit adenylate cyclase in homogenates of the ventral hypothalamus, GTP (10--100 micrometer) significantly stimulated it. Dopamine stimulated the adenylate cyclase in the hypothalamus. This action was blocked by chlorpromazine (10 micrometer) and phentolamine (100 micrometer) but not by an analogue of chlorpromazine having no neuroleptic activity or by propranolol (100 micrometer).

摘要

在存在ATP（0.6 - 3 mM）和GTP（10 - 100微摩尔）的情况下，多巴胺对大鼠脑纹状体匀浆中腺苷酸环化酶的刺激作用增强。多巴胺的刺激作用似乎是其拮抗GTP或更高浓度ATP对腺苷酸环化酶抑制作用的结果。多巴胺对该酶的刺激也依赖于MgCl2，并且在MgCl2浓度至少比ATP高两倍时达到最大值。虽然ATP在腹侧下丘脑匀浆中不抑制腺苷酸环化酶，但GTP（10 - 100微摩尔）能显著刺激它。多巴胺刺激下丘脑的腺苷酸环化酶。这种作用被氯丙嗪（10微摩尔）和酚妥拉明（100微摩尔）阻断，但不被没有抗精神病活性的氯丙嗪类似物或普萘洛尔（100微摩尔）阻断。