Department of Chemistry, Durham University, University Science Laboratories, South Road, Durham DH1 3LE, UK.
Chem Commun (Camb). 2020 Jun 7;56(45):6114-6117. doi: 10.1039/d0cc01604g. Epub 2020 May 4.
Native chemical ligation (NCL) enables the chemical synthesis of peptides via reactions between N-terminal thiolates and C-terminal thioesters under mild, aqueous conditions at pH 7-8. Here we demonstrate quantitatively how thiol speciation at N-terminal cysteines and analogues varies significantly depending upon structure at typical pH values used in NCL.
天然化学连接（NCL）能够在温和的水性条件下（pH 值为 7-8）通过 N 端硫醇盐和 C 端硫酯之间的反应，实现肽的化学合成。在此，我们定量地展示了 N 端半胱氨酸和类似物的硫醇特异性如何根据 NCL 中常用的典型 pH 值下的结构而显著变化。
